Beyaz Ramazan, Sancak Cengiz, Yildiz Çiğdem, Kuşvuran Şebnem, Yildiz Mustafa
Ahi Evran University, Faculty of Agriculture, Department of Soil Science and Plant Nutrition, Bagbası, Kırşehir 40100, Turkey.
Ankara University, Faculty of Agriculture, Department of Field Crops, Dıskapı, Ankara 06110, Turkey.
Appl Radiat Isot. 2016 Dec;118:73-79. doi: 10.1016/j.apradiso.2016.09.005. Epub 2016 Sep 6.
Effects of gamma radiation on physiological responses of the M sainfoin plants were investigated. Seeds of sainfoin ecotype 'Koçaş' were exposed to 0, 400, 500 and 600Gy from a Co source at a dose rate of 0.483 kGyh. Irradiated and unirradiated seeds were sown into culture vessels containing MS-basal medium to be cultured for 30 days under in vitro conditions. At the end of this period, seedlings, which germinated from the radiated and unirradiated seeds, were transferred into pots in a growth chamber for 30 days more. Chlorophyll contents, activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR), as well as contents of monodehydroascorbate reductase (MDA) and proline were examined in unirradiated and irradiated 60-day-old seedlings. Overall, the activities of the antioxidant enzymes (SOD, CAT and GR) and contents of chlorophyll and proline in the leaves tended to increase after irradiation in a dose-dependent manner. By contrast, the activity of APX decreased. The lipid peroxidation characterized by the MDA content remained unchanged, except after irradiation to 500Gy. The highest CAT activity and the highest proline content were observed after irradiation to the highest dose of 600Gy. The highest SOD and GR activities were observed after irradiation to the lowest tested dose of 400Gy. This is the first study that provided basic information on the impact of gamma radiation on physiological responses of sainfoin and its radiosensitivity. These findings will be useful in development of a mutation breeding program of sainfoin.
研究了γ辐射对红豆草植株生理反应的影响。红豆草生态型‘科恰什’的种子以0.483 kGy/h的剂量率接受来自钴源的0、400、500和600 Gy辐射。将辐照和未辐照的种子播种到含有MS基本培养基的培养容器中，在体外条件下培养30天。在此期间结束时，将从辐照和未辐照种子中萌发的幼苗转移到生长室的花盆中再培养30天。检测了未辐照和辐照60日龄幼苗的叶绿素含量、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）和谷胱甘肽还原酶（GR）的活性，以及单脱氢抗坏血酸还原酶（MDA）和脯氨酸的含量。总体而言，辐照后叶片中抗氧化酶（SOD、CAT和GR）的活性以及叶绿素和脯氨酸的含量呈剂量依赖性增加。相比之下，APX的活性降低。以MDA含量为特征的脂质过氧化作用除在500 Gy辐照后外保持不变。在600 Gy最高剂量辐照后观察到最高的CAT活性和最高的脯氨酸含量。在400 Gy最低测试剂量辐照后观察到最高的SOD和GR活性。这是第一项提供γ辐射对红豆草生理反应影响及其辐射敏感性基本信息的研究。这些发现将有助于红豆草突变育种计划的制定。
