Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, Espoo, Finland.
Bioresour Technol. 2013 Apr;133:522-8. doi: 10.1016/j.biortech.2013.01.175. Epub 2013 Feb 8.
Sequential fractionation of extractive-free maize stems was carried out using two mild alkaline extractions (0.5 and 2 M NaOH, 20°C, 24h) before and after endoglucanase treatment. This procedure provided two lignin-carbohydrate fractions (LC1 and LC2) recovered after each alkali treatment. LC1 and LC2 contained 39% and 8% of the total lignin amount, respectively. These two fractions contained structurally distinct lignin molecules. While the content of resistant interunit bonds in lignin was 77% in LC1, it was increased up to 98% in LC2. Not unexpectedly, both alkali-soluble fractions contained substantial amount of p-coumaric and ferulic acids ether-linked to lignins. These results outline heterogeneity of maize stem lignins related to fractionation of grass materials.
采用两种温和的碱性提取（0.5 和 2 M NaOH，20°C，24 h）对无提取剂的玉米秸秆进行顺序分段提取，在进行内切葡聚糖酶处理前后进行。该过程提供了两种木质素-碳水化合物级分（LC1 和 LC2），分别在每次碱处理后回收。LC1 和 LC2 分别含有 39%和 8%的总木质素含量。这两个级分含有结构不同的木质素分子。虽然 LC1 中木质素的抗性单元间键含量为 77%，但在 LC2 中增加到 98%。不出所料，两种碱溶性级分都含有大量与木质素相连的 p-香豆酸和阿魏酸醚。这些结果概述了与草材料分级相关的玉米秸秆木质素的异质性。
