Key Laboratory of Animal Molecular Nutrition of Education of Ministry, College of Animal Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, China.
Arch Microbiol. 2013 May;195(5):349-56. doi: 10.1007/s00203-013-0877-7. Epub 2013 Mar 2.
Depending on the microenvironment, macrophages can acquire distinct functional phenotypes, referred to as classically activated M1 and M2. M1 macrophages are considered potent effector cells that kill intracellular pathogens, and M2 macrophages promote the resolution of wound healing. In this study, we are interested to know whether probiotic Bacillus amyloliquefaciens (Ba) can induce macrophages polarization. Real-time fluorescence PCR analysis demonstrated that the expression of IL-1β, iNOS, TNF-α and IL-6 genes for M1 macrophages was significantly increased at 1.5 h after probiotic Ba treatment compared to the probiotic Ba-free treatment (P < 0.01), whereas the expression of M2 macrophage marker genes (Arg1, Fizz1, MR, Ym1) was decreased (P < 0.05). Furthermore, the phagocytic activity was dramatically increased in the Ba-treated BMDMs using a FITC-dextran endocytosis assay. Together, these findings indicated that probiotic Ba facilitated polarization of M1 macrophages and enhanced its phagocytic capacity. The results expanded our knowledge about probiotic function-involved macrophage polarization.
根据微环境的不同，巨噬细胞可以获得不同的功能表型，被称为经典激活的 M1 和 M2。M1 巨噬细胞被认为是杀死细胞内病原体的有效效应细胞，而 M2 巨噬细胞则促进伤口愈合的解决。在这项研究中，我们有兴趣了解益生菌解淀粉芽孢杆菌 (Ba) 是否可以诱导巨噬细胞极化。实时荧光 PCR 分析表明，与无益生菌 Ba 处理相比，益生菌 Ba 处理后 1.5 小时 M1 巨噬细胞的 IL-1β、iNOS、TNF-α 和 IL-6 基因表达显著增加（P<0.01），而 M2 巨噬细胞标记基因（Arg1、Fizz1、MR、Ym1）的表达则降低（P<0.05）。此外，通过 FITC-葡聚糖内吞测定法，在用 Ba 处理的 BMDMs 中，吞噬活性显著增加。总之，这些发现表明益生菌 Ba 促进了 M1 巨噬细胞的极化，并增强了其吞噬能力。这些结果扩展了我们对益生菌功能相关的巨噬细胞极化的认识。
