Plant Metabolic Engineering Laboratory, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur, 176061 Himachal Pradesh, India.
Biochem Genet. 2013 Aug;51(7-8):493-502. doi: 10.1007/s10528-013-9580-2. Epub 2013 Feb 28.
DNA methylation is known as an epigenetic modification that affects gene expression in plants. Variation in CpG methylation behavior was studied in two natural horse gram (Macrotyloma uniflorum [Lam.] Verdc.) genotypes, HPKC2 (drought-sensitive) and HPK4 (drought-tolerant). The methylation pattern in both genotypes was studied through methylation-sensitive amplified polymorphism. The results revealed that methylation was higher in HPKC2 (10.1%) than in HPK4 (8.6%). Sequencing demonstrated sequence homology with the DRE binding factor (cbf1), the POZ/BTB protein, and the Ty1-copia retrotransposon among some of the polymorphic fragments showing alteration in methylation behavior. Differences in DNA methylation patterns could explain the differential drought tolerance and the epigenetic signature of these two horse gram genotypes.
DNA 甲基化是一种表观遗传修饰，它影响植物中的基因表达。在两个天然马豆（Macrotyloma uniflorum [Lam.] Verdc.）基因型，HPKC2（干旱敏感）和 HPK4（耐旱）中，研究了 CpG 甲基化行为的变异。通过甲基化敏感扩增多态性研究了两种基因型的甲基化模式。结果表明，HPKC2 中的甲基化程度（10.1%）高于 HPK4（8.6%）。测序表明，一些表现出甲基化行为改变的多态性片段与 DRE 结合因子（cbf1）、POZ/BTB 蛋白和 Ty1-copia 反转录转座子具有序列同源性。DNA 甲基化模式的差异可以解释这两个马豆基因型的差异耐旱性和表观遗传特征。
