Amin Maha M, El-Hawary Amira K, Farouk Omar
Department of Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Indian J Pathol Microbiol. 2012 Oct-Dec;55(4):456-60. doi: 10.4103/0377-4929.107780.
In breast cancer, the expression of CD117 represents a highly controversial subject but the majority of studies have found decreased c-kit expression in malignant breast epithelium. A number of studies have reported that increased intratumoral microvessel density (MVD) is associated with poor prognosis in breast cancer. The aim of the study was to assess the relation of CD117 and MVD with other clinicopathological parameters in invasive breast carcinomas using the tissue microarray technique.
A total of 126 cases of invasive breast carcinoma of different histological types and grades were collected from files of a pathology department during 2010. Clinicopathological and histological parameters were evaluated. Sections from formalin-fixed, paraffin-embedded tumor tissues microarray blocks were immunostained with CD117 and CD34. Statistical analysis of data was done using SPSS, version 16.0.
About 29% of invasive breast carcinomas were CD117 positive. There were significant differences between expression of CD117 in the tumor epithelial cells and age of the patient; tumor grade; tumor size, and LN metastasis. Also, there was significant relation between expression of CD117 in the tumor epithelial cells and MVD, expression of estrogen, and progesterone receptors. On multivariate analysis, the most important predictors of negativity of CD117 were tumor size and positive lymph node involvement.
Lack of CD117 immunoreactivity in invasive breast carcinoma was associated with features of more aggressive tumor behavior as higher microvessel density, larger size, higher tumor grade, more lymph node metastasis, and negative estrogen and progesterone receptors.
在乳腺癌中，CD117的表达是一个极具争议的话题，但大多数研究发现恶性乳腺上皮中c-kit表达降低。多项研究报告称，肿瘤内微血管密度（MVD）增加与乳腺癌预后不良相关。本研究旨在利用组织芯片技术评估浸润性乳腺癌中CD117和MVD与其他临床病理参数的关系。
2010年从某病理科档案中收集了126例不同组织学类型和分级的浸润性乳腺癌病例。对临床病理和组织学参数进行评估。用CD117和CD34对福尔马林固定、石蜡包埋的肿瘤组织芯片块切片进行免疫染色。使用SPSS 16.0版对数据进行统计分析。
约29%的浸润性乳腺癌CD117呈阳性。肿瘤上皮细胞中CD117的表达与患者年龄、肿瘤分级、肿瘤大小和淋巴结转移之间存在显著差异。此外，肿瘤上皮细胞中CD117的表达与MVD、雌激素和孕激素受体的表达之间存在显著关系。多因素分析显示，CD117阴性的最重要预测因素是肿瘤大小和阳性淋巴结受累情况。
浸润性乳腺癌中CD117免疫反应性的缺乏与更具侵袭性的肿瘤行为特征相关，如更高的微血管密度、更大的肿瘤大小、更高的肿瘤分级、更多的淋巴结转移以及雌激素和孕激素受体阴性。
