Department of Immunobiology, Maria Curie-Sklodowska University, Lublin, Poland.
J Basic Microbiol. 2014 Mar;54(3):232-46. doi: 10.1002/jobm.201200273. Epub 2013 Mar 4.
The influence of infection with an entomopathogenic strain of Pseudomonas aeruginosa on Galleria mellonella hemocytes was investigated. Extensive bacteriaemia developed 18 h after infection. This was correlated with significant changes in morphology, viability and the spreading ability of immunocompetent hemocytes, namely granulocytes and plasmatocytes. Since bacteriaemia developed, membrane blebbing, cytoplasm vacuolization, cell and organelle swelling, and chromatin condensation were observed among others. These features are typical for apoptotic and autophagal cell death. A gradually increasing level of procaspase and its activation as well as lack of DNA degradation were also detected. Propidium iodide and acridine orange staining indicated that hemocytes become dead ultimately. Infection of G. mellonella larvae with P. aeruginosa also caused significant changes in the arrangement of the actin cytoskeleton in the hemocytes, which might be correlated with their restricted spreading ability.
研究了昆虫病原假单胞菌对欧洲粉螟血淋巴细胞的影响。感染 18 小时后，发生了广泛的菌血症。这与免疫活性血淋巴细胞（即粒细胞和浆细胞）的形态、活力和扩散能力的显著变化相关。由于发生了菌血症，观察到细胞膜起泡、细胞质空泡化、细胞和细胞器肿胀以及染色质浓缩等现象。这些特征是典型的凋亡和自噬细胞死亡。还检测到逐渐增加的半胱天冬酶水平及其激活以及缺乏 DNA 降解。碘化丙啶和吖啶橙染色表明，血淋巴细胞最终死亡。绿脓杆菌感染欧洲粉螟幼虫也导致血淋巴细胞中肌动蛋白细胞骨架的排列发生显著变化，这可能与其扩散能力受限有关。
