Technova Corporation, 1926 Turner Street, Lansing, Michigan 48906, USA.
Biotechnol Bioeng. 2013 Aug;110(8):2123-30. doi: 10.1002/bit.24883. Epub 2013 Mar 26.
Cellulase, xylanase, and β-glucosidase production was studied on novel nano-shear pretreated corn stover by the mixed fungi culture. The high shear force from a modified Tayor-Couette nano-shear mixing reactor efficiently disintegrated corn stover, resulting in a homogeneous watery mash with particles in much reduced size. Scanning electron microscope study showed visible mini-pores on the fiber cell wall surface, which could improve the accessibility of the pretreated corn stover to microorganisms. Mixed fungal culture of Trichoderma reesei RUT-C30 and Aspergillus niger produced enzymes with higher cellulolytic and xylanolytic activities on corn stover pretreated with nano-shear mixing reactor, in comparison with other pretreatment methods, including acid and ammonia fiber explosion (AFEX) pretreatment. The hydrolytic potential of the whole fermentation broth from the mixed fungi was studied, and the possibility of applying the whole cell saccharification concept was also investigated to further reduce the cost of lignocellulose hydrolysis.
研究了新型纳米剪切预处理玉米秸秆的混合真菌培养物中纤维素酶、木聚糖酶和β-葡萄糖苷酶的生产。改良的泰勒-库埃特纳米剪切混合反应器中的高剪切力有效地将玉米秸秆分解，得到颗粒尺寸大大减小的均匀水性糊状物。扫描电子显微镜研究表明，纤维细胞壁表面有可见的微孔，这可以提高预处理玉米秸秆对微生物的可及性。与其他预处理方法（包括酸和氨纤维爆炸（AFEX）预处理）相比，里氏木霉 RUT-C30 和黑曲霉混合真菌培养物在经过纳米剪切混合反应器预处理的玉米秸秆上产生了更高的纤维素酶和木聚糖酶活性的酶。研究了混合真菌整个发酵液的水解潜力，并探讨了应用全细胞糖化概念的可能性，以进一步降低木质纤维素水解的成本。
