Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University Richland, WA 99354, United States.
Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University Richland, WA 99354, United States.
Bioresour Technol. 2014 Feb;154:282-9. doi: 10.1016/j.biortech.2013.12.059. Epub 2013 Dec 24.
Cellulase production by two filamentous fungi Trichoderma reesei RUT-C30 and novel fungal strain, Aspergillus saccharolyticus on pretreated corn stover was investigated. Cellulase production was followed by the hydrolysis of two feedstocks, wet-exploded corn stover (WECS) and wet-exploded loblolly pine (WELP) by on-site produced enzyme cocktails containing cellulase from T. reesei RUT-C30 and β-glucosidase from A. saccharolyticus. The sugar yields using the on-site enzyme cocktails were compared with commercial enzymes preparations, Celluclast 1.5L and Novozym 188 at two substrate concentrations, 5% and 10% (w/w) and enzyme loading at 5 and 15 FPU/g glucan for WECS and WELP. The highest sugar yields were obtained at 5% (w/w) substrate concentration and 15 FPU/g glucan for both feedstocks. Glucose yields of 81% and 88% were obtained from on-site and commercial enzymes, respectively using WECS as feed stock. The sugar yields were 55% and 58% for WELP samples hydrolyzed with on-site and commercial enzymes, respectively.
研究了两种丝状真菌里氏木霉（Trichoderma reesei RUT-C30）和新型真菌解淀粉曲霉（Aspergillus saccharolyticus）在预处理玉米秸秆上的纤维素酶生产。通过现场生产的含有里氏木霉（Trichoderma reesei RUT-C30）的纤维素酶和解淀粉曲霉（Aspergillus saccharolyticus）的β-葡萄糖苷酶的酶混合物，对两种原料（湿挤压玉米秸秆（WECS）和湿挤压火炬松（WELP））进行水解，以跟踪纤维素酶的生产。使用现场酶混合物的糖收率与两种底物浓度（5%和 10%（w/w））和酶用量（5 和 15 FPU/g 葡聚糖）下的商业酶制剂 Celluclast 1.5L 和 Novozym 188 进行了比较。在两种原料中，在 5%（w/w）的底物浓度和 15 FPU/g 葡聚糖下均获得了最高的糖收率。使用 WECS 作为原料，现场酶和商业酶分别获得了 81%和 88%的葡萄糖收率。对于 WELP 样品，用现场酶和商业酶分别水解得到 55%和 58%的糖收率。
