Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, P.R. China.
J Basic Microbiol. 2013 Nov;53(11):942-52. doi: 10.1002/jobm.201200200. Epub 2013 Mar 1.
While much is known about the microbial diversity in some hypersaline environments, little is known about those of salt mine tunnel soils. The objective of this study was to conduct a comprehensive phylogenetic comparison of the archaeal and bacterial communities present in Yipinglang salt mine (YPL) and Qiaohou salt mine (QH) tunnels differing in salinity and salt composition using 16S rRNA gene clone libraries. Two hundred twenty-eight sequences for QH and 182 sequences for YPL were analyzed by amplified ribosomal DNA-restriction analysis. Libraries revealed 44 bacterial and 57 archaeal different operational taxonomic units belonging to at least 8 bacterial and 3 archaeal divisions, but not all divisions were observed in both salt mines. The bacterial community affiliated with the Bacteroidetes was the most abundant (60% of clones) in QH, while the community in YPL was dominated by δ-Proteobacteria (45% of clones). All archaeal clones from QH were affiliated with Halobacteriaceae. In contrast, in the YPL library, 49% of clones belonged to Halobacteriaceae, 31% of clones related to unclassified archaea, and 21% of clones belonged to Crenarchaeota. Bioinformatic analysis and comparisons showed that the clone libraries were significantly different between two salt mines.
虽然人们对一些高盐环境中的微生物多样性有了很多了解，但对盐矿隧道土壤中的微生物多样性却知之甚少。本研究的目的是通过 16S rRNA 基因克隆文库，对盐度和盐成分不同的一平浪盐矿（YPL）和桥头盐矿（QH）隧道中的古菌和细菌群落进行全面的系统发育比较。通过扩增核糖体 DNA 限制分析对 QH 的 228 个序列和 YPL 的 182 个序列进行了分析。文库揭示了 44 个细菌和 57 个古菌不同的操作分类单元，属于至少 8 个细菌和 3 个古菌门，但并非所有门都在两个盐矿中都有观察到。与拟杆菌门（Bacteroidetes）相关的细菌群落在 QH 中最为丰富（占克隆的 60%），而 YPL 中的群落则以 δ-变形菌门（δ-Proteobacteria）为主（占克隆的 45%）。QH 中的所有古菌克隆都与盐杆菌科（Halobacteriaceae）有关。相比之下，在 YPL 文库中，49%的克隆属于盐杆菌科，31%的克隆与未分类古菌有关，21%的克隆属于泉古菌门（Crenarchaeota）。生物信息学分析和比较表明，两个盐矿的克隆文库存在显著差异。
