Chen Shaoxing, Sun Siqi, Xu Yao, Lv Jinting, Chen Linan, Liu Liu
College of Life Sciences, Anhui Normal University, No.1 Beijing East Road, Wuhu, 241000, Anhui, China.
College of Life Sciences, Honghe University, No.1 Xuefu Road, Mengzi, 661100, Yunnan, China.
Curr Microbiol. 2018 Jun;75(6):677-683. doi: 10.1007/s00284-018-1432-1. Epub 2018 Jan 10.
A non-motile, pleomorphic rod-shaped or oval, red-pigmented (nearly scarlet), extremely halophilic archaeon, strain Y78, was isolated from a salt deposit of Yunnan salt mine, China. Analysis of the 16S rRNA gene sequence showed that it was phylogenetically related to species of the genus Halorubrum, with a close relationship to Halorubrum rutilum YJ-18-S1 (98.6%), Halorubrum yunnanense Q85 (98.3%), and Halorubrum lipolyticum 9-3 (98.1%). The temperature, NaCl, and pH ranges for growth were 25-50 °C, 12-30% (w/v), and 6.5-9.0, respectively. Mg was required for growth. The polar lipids of strain Y78 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, and a sulfated diglycosyl diether. The DNA G+C content was 66.6 mol%. DNA-DNA hybridization values between strain Y78 and two closely related species of the genus Halorubrum were far below 70%. Based on the data presented in this study, strain Y78 represents a novel species for which the name Halorubrum depositum sp. nov. is proposed; the type strain is Y78 (= CGMCC 1.15456 = JCM 31272).
从中国云南盐矿的盐沉积物中分离出一株不运动、多形态的杆状或椭圆形、红色色素（近乎猩红色）、极端嗜盐古菌，菌株Y78。16S rRNA基因序列分析表明，它在系统发育上与嗜盐红菌属的物种相关，与嗜盐红菌YJ-18-S1（98.6%）、云南嗜盐红菌Q85（98.3%）和嗜盐解脂红菌9-3（98.1%）关系密切。生长的温度、NaCl和pH范围分别为25-50°C、12-30%（w/v）和6.5-9.0。生长需要镁。菌株Y78的极性脂质为磷脂酰甘油、磷脂酰甘油磷酸甲酯、磷脂酰甘油硫酸酯和一种硫酸化二糖基二醚。DNA G+C含量为66.6 mol%。菌株Y78与嗜盐红菌属的两个密切相关物种之间的DNA-DNA杂交值远低于70%。基于本研究提供的数据，菌株Y78代表一个新物种，为此提出名称嗜盐红菌盐沉积物新种；模式菌株为Y78（=CGMCC 1.15456=JCM 31272）。
