Takeuchi Hiroya, Kawakubo Hirofumi, Saikawa Yoshiro, Omori Tai, Kitagawa Yuko
Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
Nihon Geka Gakkai Zasshi. 2013 Jan;114(1):9-12.
Hematoxylin and eosin (HE) and immunohistochemical (IHC) staining have been commonly used in combination with thin serial sectioning of frozen and paraffin-embedded specimens for the detection of micrometastatic disease in the lymph nodes of patients with esophageal cancer. The application of IHC has markedly improved the sensitivity of micrometastatic disease detection in the lymph nodes beyond the capability of routine HE staining alone. Increasing the sensitivity of occult tumor cell detection in the lymph nodes using molecular-based analysis should enable a more accurate understanding of the clinical significance of various patterns of micrometastatic nodal disease. Further studies will explore the clinical significance of micrometastases or isolated tumor cells in the lymph nodes in patients with esophageal cancer. Individualized selective and modified lymphadenectomy targeting sentinel nodes for clinically NO early esophageal cancer may become feasible and clinically useful as a less invasive surgical procedure.
苏木精和伊红（HE）染色以及免疫组织化学（IHC）染色通常与冷冻和石蜡包埋标本的薄连续切片相结合，用于检测食管癌患者淋巴结中的微转移疾病。免疫组织化学的应用显著提高了淋巴结中微转移疾病检测的灵敏度，这是常规HE染色单独无法做到的。使用基于分子的分析提高淋巴结中隐匿肿瘤细胞检测的灵敏度，应能更准确地理解各种微转移淋巴结疾病模式的临床意义。进一步的研究将探索食管癌患者淋巴结中微转移或孤立肿瘤细胞的临床意义。针对临床NO期早期食管癌的前哨淋巴结进行个体化选择性改良淋巴结清扫术，作为一种侵入性较小的手术，可能会变得可行且具有临床实用性。
