Livanova N N, Tikunova N V, Livanov S G, Fomenko N V
Parazitologiia. 2012 Sep-Oct;46(5):340-9.
Ticks of the genus Ixodes were collected in 2010 in the lowland part of Toguchinsk district of Novosibirsk Province (Russia) and in the forest-park area of Novosibirsk Scientific Centre and its outskirts (Sovetskiy district of Novosibirsk), and identified as Ixodes persulcatus (Schulze, 1930) (18 females and 13 males) and Ixodes pavlovskyi (13 females and 10 males). Ten specimens of each sex from each collecting site were examined. The following nine characters were used: the length and width of the scutum (conscutum) and of the gnathosoma in ventral view; the length of palpal segments II-III; the width of the hypostome; the length of idiosoma with scapula, of leg I, of the medial spur on fore coxa (Taiga..., 1985; Filippova, Musatov, 1996; Filippova, Panova, 1998). According to morphometric characters, specimens of Ixodes pavlovskyi collected in the forest-park area of the Novosibirsk Scientific Centre were identified as the subspecies I. p. occidentalis Filippova et Panova, 1998. Nucleotide sequences of the COI mitochondrial gene fragment were determined for 56 ticks. Phylogenetic analysis of the COI gene fragment in representatives of the persulcatus-ricinus species-group dwelling in Asia demonstrated high degree of conservatism. Molecular-genetic methods allow reliable identification of morphologically similar species I. pavlovskyi and I. persulcatus, pathogenic for humans.
2010年，在俄罗斯新西伯利亚州托古钦斯克区的低地部分以及新西伯利亚科学中心及其郊区（新西伯利亚苏维埃区）的森林公园区域采集了硬蜱属蜱虫，并鉴定为全沟硬蜱（舒尔策，1930年）（18只雌性和13只雄性）和帕氏硬蜱（13只雌性和10只雄性）。对每个采集地点的每种性别的10个标本进行了检查。使用了以下九个特征：盾板（consutum）和口器腹面观的长度和宽度；须肢第二节至第三节的长度；下咽的宽度；有肩胛的虫体长度、第一对足的长度、前足基节内侧距的长度（《泰加……》，1985年；菲利波娃、穆萨托夫，1996年；菲利波娃、帕诺娃，1998年）。根据形态测量特征，在新西伯利亚科学中心森林公园区域采集的帕氏硬蜱标本被鉴定为1998年菲利波娃和帕诺娃命名的西方亚种I. p. occidentalis。测定了56只蜱虫的细胞色素氧化酶亚基I（COI）线粒体基因片段的核苷酸序列。对亚洲全沟硬蜱-蓖麻硬蜱物种组代表的COI基因片段进行系统发育分析，结果显示高度保守。分子遗传学方法能够可靠地鉴定形态相似但对人类致病的帕氏硬蜱和全沟硬蜱。
