Livanova Natalia N, Tikunov Artem Yu, Kurilshikov Alexander M, Livanov Stanislav G, Fomenko Nataliya V, Taranenko Dmitrii E, Kvashnina Anna E, Tikunova Nina V
Institute of Systematics and Ecology of Animals Siberian Branch of the Russian Academy of Sciences, Frunze Street 11, 630091, Novosibirsk, Russia.
Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences, Prosp. Lavrent'eva 8, Novosibirsk, Russia.
Exp Appl Acarol. 2015 Nov;67(3):441-56. doi: 10.1007/s10493-015-9947-7. Epub 2015 Jul 23.
The most epidemiologically significant tick species in Siberia involved in transmission of a large number of pathogens causing human infectious diseases is Ixodes persulcatus. Ixodes pavlovskyi, being more active, also poses epidemiological threats. These tick species share morphology, activity seasons and geographic distribution range. In this paper, we characterize the geographic and genetic structures of I. persulcatus and I. pavlovskyi populations inhabiting the southern part of Western Siberia (Russia and Kazakhstan)--the western part of I. pavlovskyi distribution range. The data are based on six distinct Ixodes tick populations. Analysis of the concatenated mitochondrial marker sequences (16S rRNA and COI) and the nuclear sequence (ITS2) showed genetic polymorphisms in both I. persulcatus and I. pavlovskyi ticks inhabiting the sympatric zone. We could not determine the phylogeographic structure of I. pavlovskyi populations whereas for I. persulcatus significant within-region variance was shown. Notably, the abundance of I. persulcatus ticks negatively correlates with nucleotide and haplotype diversity in the concatenated sequence of mitochondrial gene (16S rRNA and COI) fragments. This is the first description of the genetic polymorphism of I. persulcatus and I. pavlovskyi ticks coexisting in a sympatric zone based on analysis of mitochondrial and nuclear markers.
在西伯利亚，参与传播大量导致人类传染病病原体的、在流行病学上最重要的蜱种是全沟硬蜱。更为活跃的帕氏硬蜱也构成流行病学威胁。这些蜱种在形态、活动季节和地理分布范围上有共同之处。在本文中，我们描述了栖息于西西伯利亚南部（俄罗斯和哈萨克斯坦）——帕氏硬蜱分布范围西部的全沟硬蜱和帕氏硬蜱种群的地理和遗传结构。数据基于六个不同的硬蜱种群。对串联的线粒体标记序列（16S rRNA和COI）和核序列（ITS2）的分析表明，栖息于同域分布区的全沟硬蜱和帕氏硬蜱均存在遗传多态性。我们无法确定帕氏硬蜱种群的系统地理学结构，而全沟硬蜱显示出显著的区域内差异。值得注意的是，全沟硬蜱的丰度与线粒体基因（16S rRNA和COI）片段串联序列中的核苷酸和单倍型多样性呈负相关。这是基于线粒体和核标记分析对同域分布区共存的全沟硬蜱和帕氏硬蜱的遗传多态性的首次描述。
