Kudva Indira T, Biernbaum Erika N, Trachsel Julian M
United States of America Department of Agriculture, Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center, Agricultural Research Service, Ames, Iowa United States.
Oak Ridge Institute for Science and Education (ORISE), ARS Research Participation Program, Oak Ridge, Tennessee, United States.
PLoS One. 2025 Jun 4;20(6):e0313978. doi: 10.1371/journal.pone.0313978. eCollection 2025.
This study demonstrates that non-O157 serotypes O26:H11, O111:H8, O145:NM have similar survival and protein expression patterns in rumen fluid with variations being influenced primarily by rumen fluid composition associated with diet. Unlike STEC O157, under in vivo conditions, the growth dynamics of the non-O157 serotypes were comparable to that of non-STEC, commensal E. coli. Hence, exploring bacterial protein expression within the host is critical in discerning therapeutic targets, unique to/shared between STEC, for broad control strategies. In addition, this study further validates the value of using a non-terminal animal model for rumen studies that reduces number of animals used for an experiment.
本研究表明，非O157血清型O26:H11、O111:H8、O145:NM在瘤胃液中具有相似的存活和蛋白质表达模式，其差异主要受与饮食相关的瘤胃液成分影响。与肠出血性大肠杆菌O157不同，在体内条件下，非O157血清型的生长动态与非肠出血性大肠杆菌、共生大肠杆菌相当。因此，探索宿主内细菌的蛋白质表达对于识别肠出血性大肠杆菌特有的/共有的治疗靶点以制定广泛的控制策略至关重要。此外，本研究进一步验证了使用非终末动物模型进行瘤胃研究的价值，该模型可减少实验所用动物的数量。
