Anal Chem. 2013 Apr 2;85(7):3497-500. doi: 10.1021/ac4000915. Epub 2013 Mar 11.
Free flow electrophoresis is used for rapid and high-recovery isolation of homogeneous preparations of functionally active membrane proteins inserted into nanodiscs. The approach enables isolation of integral and membrane anchored proteins and is also applicable following introduction of, e.g., fluorescent tags. Preparative separation of membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates results in monodisperse nanodisc preparations ideal for structural and functional characterization using biophysical methods.
自由流电泳可用于快速且高回收率地分离插入纳米盘的功能活性膜蛋白的均一制剂。该方法能够分离完整的和膜锚定的蛋白质，并且在引入例如荧光标记物之后也适用。负载有膜蛋白的纳米盘与空纳米盘和蛋白质聚集体的制备性分离导致单分散纳米盘制剂，非常适合使用生物物理方法进行结构和功能表征。
