College of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, Jiangsu, 22509, China.
Gene. 2013 May 15;520(2):148-55. doi: 10.1016/j.gene.2013.02.026. Epub 2013 Feb 28.
The heat shock 90/70 organizing protein (Hop), also known as Sti-1 (stress-induced protein-1), is a co-chaperone that usually mediates the interaction of Hsp90 and Hsp70 and has been extensively characterized in mammals and plants. However, its role in insects remains unknown. In the present study, we isolated and characterized a Hop homologue gene from Frankliniella occidentalis (Fohop). The Fohop contains a 1659bp ORF encoding a protein of 552 amino acids with a caculated molecular mass of approximately 62.25kDa, which displays a reasonable degree of identity with the known Hops and shares several canonical motifs, including three tetratricopeptide repeated motif domains (TPR1, TPR2A and TPR2B) and two aspartic acid-proline (DP) repeat motifs (DP1 and DP2). As in other hops, Fohop contains introns, but the number and the position are quite variable. The mRNA expression patterns indicated that Fohop was constitutively expressed throughout the developmental stages, but was obviously upregulated by heat stress both in larvae and adults. Our studies imply that Hop, as in other Hsps, may play an important role in heat shock response of F. occidentalis.
热休克 90/70 组织蛋白（Hop），也称为 Sti-1（应激诱导蛋白-1），是一种共伴侣，通常介导 Hsp90 和 Hsp70 的相互作用，并在哺乳动物和植物中得到了广泛的研究。然而，它在昆虫中的作用尚不清楚。在本研究中，我们从西方花蓟马（Frankliniella occidentalis）中分离并鉴定了一个 Hop 同源基因（Fohop）。Fohop 包含一个 1659bp 的 ORF，编码一个 552 个氨基酸的蛋白质，计算分子量约为 62.25kDa，与已知的 Hop 具有一定的同源性，并具有几个典型的基序，包括三个四肽重复结构域（TPR1、TPR2A 和 TPR2B）和两个天冬氨酸-脯氨酸（DP）重复基序（DP1 和 DP2）。与其他 Hop 一样，Fohop 也含有内含子，但数量和位置都非常多变。mRNA 表达模式表明，Fohop 在发育的各个阶段都持续表达，但在幼虫和成虫中受到热应激的明显上调。我们的研究表明，Hop 与其他 Hsps 一样，可能在西方花蓟马的热应激反应中发挥重要作用。
