Han Xue, Jia Yulong, Dai Changchun, Wang Xiaoyun, Liu Jian, Tian Zhenqi
Key Laboratory of Crop Pests in Northern Cold Regions of Heilongjiang Province, College of Plant Protection, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, China.
Insects. 2025 Jul 28;16(8):772. doi: 10.3390/insects16080772.
Soybean aphid, Matsumura (Hemiptera: Aphididae), is a major pest of soybean fields. While high-temperature stress induced by global warming can initially suppress aphid populations, these pests may eventually adapt, leading to more severe infestations and crop damage. Heat shock proteins (HSPs), which are upregulated in response to heat stress to protect aphid development, also confer tolerance to other abiotic stressors, including insecticides. To investigate the role of HSPs in insecticide resistance in , we analyzed the expression profiles of three genes (, , and ) following exposure to high temperatures and insecticides. Functional validation was performed using RNA interference (RNAi) to silence genes. Our results demonstrated that genes were significantly upregulated under both heat and insecticide stress conditions. Furthermore, after feeding on dsRNA of genes, mortality rates of significantly increased when exposed to imidacloprid and lambda-cyhalothrin. This study provides evidence that genes play a crucial role in mediating thermal tolerance and insecticide resistance in .
大豆蚜，松村氏（半翅目：蚜科），是大豆田的主要害虫。虽然全球变暖引起的高温胁迫最初可以抑制蚜虫种群，但这些害虫最终可能会适应，导致更严重的虫害和作物损害。热休克蛋白（HSPs）在热应激反应中上调以保护蚜虫发育，同时也赋予对包括杀虫剂在内的其他非生物胁迫的耐受性。为了研究HSPs在大豆蚜抗药性中的作用，我们分析了暴露于高温和杀虫剂后三个HSP基因（HSP70、HSP90和HSP60）的表达谱。使用RNA干扰（RNAi）沉默HSP基因进行功能验证。我们的结果表明，HSP基因在热和杀虫剂胁迫条件下均显著上调。此外，在取食HSP基因的dsRNA后，大豆蚜在接触吡虫啉和氯氟氰菊酯时死亡率显著增加。本研究提供了证据表明HSP基因在介导大豆蚜的耐热性和抗药性中起关键作用。
