Research Laboratories for Fundamental Technology of Food, Asahi Group Holdings Limited, 1-1-21 Midori, Moriya, Ibaraki 302-0106, Japan.
Food Chem Toxicol. 2013 Jun;56:214-22. doi: 10.1016/j.fct.2013.02.021. Epub 2013 Feb 28.
To examine the safety of Dietary Applephenon® (AP) in feed, Crl: CD (SD) rats of each sex were divided into four groups and given diets containing AP at 0%, 1.25%, 2.5%, or 5.0% for 90 days. All rats survived and toxic changes were not observed throughout the study. Body weight and food efficiency in the 5.0% AP group of both sexes were significantly decreased compared with that in controls. These changes were considered to be caused by the physiological effects of AP (including the inhibitory effects on pancreatic lipase activity). Slight hypertrophy in acinar cells in the parotid and submandibular glands appeared in the 2.5% and 5.0% groups. These were suggested not to be toxicological but physiologic adaptive responses to oral stimuli by the lower pH of AP-containing diets. In conclusion, dietary AP in feed, up to a maximum level of 5.0% for 90 days, given to rats did not induce toxicological effects.
为了研究膳食苹果酚（AP）在饲料中的安全性，将 Crl：CD（SD）大鼠按性别分为四组，分别给予含 0%、1.25%、2.5%或 5.0%AP 的饲料，连续喂养 90 天。所有大鼠均存活，整个研究过程中未观察到毒性变化。与对照组相比，5.0%AP 组雌雄大鼠的体重和食物效率显著降低。这些变化被认为是 AP 的生理效应（包括对胰腺脂肪酶活性的抑制作用）所致。在 2.5%和 5.0%组的腮腺和颌下腺中出现了腺泡细胞的轻微肥大。这些变化被认为不是毒性的，而是对含有 AP 的饲料较低 pH 值的口腔刺激的生理性适应反应。总之，饲料中添加 AP，连续喂养 90 天，最高水平达 5.0%，不会引起毒理学效应。
