College of Forest Resources and Environment, Nanjing Forestry University, 159, Longpan Road, Nanjing, 210037, People's Republic of China.
Mol Biol Rep. 2013 Jun;40(6):4083-90. doi: 10.1007/s11033-013-2486-6. Epub 2013 Mar 5.
Quercus acutissima is native to eastern Asia. It has a wide distribution in China and China is an important component in understanding the ecology and genetic structure of this species. Q. acutissima attained high economic value for hardwood product and can be managed as an energy tree species. To investigate the genetic variation of Q. acutissima provenances, 12 microsatellite primer pairs were used to analyze 672 trees sampled from 28 provenances of Q. acutissima in China. All of the tested microsatellite loci proved to be effective for the studied Q. acutissima provenances. The results revealed that allele numbers varied from 5 to 13 per locus, with an average of 8 alleles per locus. The mean observed heterozygosity and expected heterozygosity were 0.4927 and 0.7023, respectively. The relatedness of the provenances was studied using the arithmetic mean algorithm based on Nei's genetic distance and principal coordinates analysis. Interestingly, both approaches revealed two main groups: one consisted of the eastern Chinese provenances, and the other comprised of the western Chinese provenances. An analysis of molecular variance indicated that most genetic variation was contained within populations (84 %). The two microsatellite markers developed in this study may be employed for genetic characterization of other oak species. Considering the management or breeding programs of Q. acutissima provenances in China, we should treat each main group as a single gene resource.
中国是麻栎的原产地之一，在中国有着广泛的分布，是研究该种生态和遗传结构的重要组成部分。麻栎因木材坚硬，是高档家具和农具用材，同时也是优良的薪炭树种和能源树种而具有很高的经济价值。为了研究麻栎种源的遗传变异，本研究利用 12 对微卫星引物对来自中国 28 个种源的 672 株麻栎进行了分析。所有测试的微卫星位点均对供试麻栎种源有效。结果表明，每个位点的等位基因数为 5-13 个，平均每个位点 8 个等位基因。观测杂合度和期望杂合度分别为 0.4927 和 0.7023。利用 Nei 的遗传距离和主坐标分析的算术平均值算法研究了种源间的亲缘关系。有趣的是，这两种方法都揭示了两个主要群体：一个由中国东部种源组成，另一个由中国西部种源组成。基于分子方差分析表明，大部分遗传变异存在于种群内（84%）。本研究开发的这两个微卫星标记可用于其他栎属树种的遗传特征分析。考虑到中国麻栎种源的管理或选育计划，我们应该将每个主要群体视为单一的基因资源。
