National Engineering Laboratory of Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
Shandong Provincial Center of Forest and Grass Germplasm Resources, Jinan 250102, China.
Genes (Basel). 2022 Jul 24;13(8):1321. doi: 10.3390/genes13081321.
Carruth. is a Chinese important energy plant with high ecological and economic values. While the species chloroplast genome has been reported, its mitochondrial genome (mitogenome) is still unexplored. Here, we assembled and annotated the mitogenome, and we compared its characteristic differences with several closely related species. The mitogenome's main structure is branched with three distinguished contigs (linear molecule 1, circular molecule 2, and circular molecule 3) with 448,982 bp total length and 45.72% GC content. The mitogenome contained 51 genes, including 32 protein-coding, 16 tRNA and 3 rRNA genes. We examined codon usage, repeated sequences, genome recombination, chloroplast to mitochondrion DNA transformation, RNA editing, and synteny in the mitogenome. Phylogenetic trees based on 29 species mitogenomes clarified the species classification. Our results provided comprehensive information of mitogenome, and they are expected to provide valuable information for Fagaceae evolutionary biology and to promote the species germplasm utilization.
麻栎是中国重要的能源植物，具有很高的生态和经济价值。虽然该物种的叶绿体基因组已经报道过，但线粒体基因组（mitogenome）仍未被探索。在这里，我们组装并注释了线粒体基因组，并与几个近缘物种进行了特征差异比较。线粒体基因组的主要结构是分支的，有三个不同的片段（线性分子 1、圆形分子 2 和圆形分子 3），总长度为 448,982 bp，GC 含量为 45.72%。线粒体基因组包含 51 个基因，包括 32 个蛋白质编码基因、16 个 tRNA 基因和 3 个 rRNA 基因。我们检查了密码子使用、重复序列、基因组重组、叶绿体到线粒体 DNA 的转化、RNA 编辑和基因排列。基于 29 种物种线粒体基因组的系统发育树阐明了物种分类。我们的研究结果提供了线粒体基因组的综合信息，预计将为壳斗科进化生物学提供有价值的信息，并促进物种种质资源的利用。
