Rauch F H, Schleicher A, Zilles K
Anatomisches Institut, Universität Köln, Federal Republic of Germany.
Anat Embryol (Berl). 1990;181(4):373-9. doi: 10.1007/BF00186909.
The neuronal profiles of cell bodies in the facial nerve nucleus of adult rats were measured after partial peripheral nerve transsection. An image analyser was used for automatic recognition and morphometry. Based on classification of the neuronal profiles described in a previous paper, a morphometrical study was performed in order to analyse structural aspects of the retrograde reaction in quantitative terms. Beside the quantification of classical features of retrograde reaction (chromatolysis; nuclear eccentricity; increased basophilia; perikaryal, nuclear and nucleolar swelling), several reactive changes so far not recognized (karyoplasmic basophilia and granulation) were detected. These changes were interpreted as morphological correlates of regeneration in retrogradely reacting cells.
在成年大鼠面神经核进行部分外周神经横断后，对细胞体的神经元轮廓进行了测量。使用图像分析仪进行自动识别和形态测量。基于先前一篇论文中描述的神经元轮廓分类，进行了一项形态测量研究，以便从定量角度分析逆行反应的结构方面。除了对逆行反应的经典特征（染色质溶解；核偏心；嗜碱性增加；胞体、细胞核和核仁肿胀）进行量化外，还检测到了一些迄今未被认识到的反应性变化（核质嗜碱性和颗粒化）。这些变化被解释为逆行反应细胞中再生的形态学关联。
