Ostrowski K, Kieler J, Balslev I
Department of Histology, Medical Academy in Warsaw, Poland.
Anticancer Res. 1990 Mar-Apr;10(2A):359-67.
Eight human urothelial cell lines established and propagated in the Fibiger Institute, differing in their transformation grade as described by Christensen et al were brought into suspension and contacted with a panel of seven substrates, ie. Con. A, Fibronectin, WGA, Collagen IV, Laminin, PNA and artificial basal membrane. Cell adhesion was measured on big cell populations with the use of flow cytophotometry, measured samples contained nonadhering cells and fluorescent beads, added as an internal standard. It was found that the cell lines differed from each other by the kinetics of cell adhesion to the chosen panel. A higher percentage of cells belonging to established cell lines adhered to PNA, WGA or Con A coated surfaces than to laminin, fibronectin or collagen IV. The difference in adherence of cells to fibronectin, laminin or collagen IV coated surfaces makes differentiation between the single cell lines possible. Discriminant analysis of the total patterns of adhesion of all cell lines was used for comparison. The effect of trypsinization on the structure of the cell surface, accompanied by possible changes in adherence, was evaluated by comparison of the immediate results with those obtained with cells after 3 hours of recovery at 37 degrees C. It was found that all cell lines showed a better adherence to laminin after recovery. This is consistent with the published data on the high sensitivity of laminin receptors to trypsinization. On the other hand, the receptors for PNA, WGA and Con A were less expressed after the recovery period, as evidenced by a decreased adherence of the cells to these three substrates. The effect of recovery of cells on the amount of their receptors for fibronectin and collagen IV was different in different cell lines, but the direction of change for this pair of receptors was identical in a given cell line. In some instances the recovery period had no effect whatsoever on cell adherence. The different pattern of adhesion of cells belonging to established cell lines to the chosen panel of substrates can be used as a parameter for characterization of single cell lines, but it does not correlate with their grade of transformation.
在菲比格研究所建立并传代培养的8种人尿路上皮细胞系，如克里斯滕森等人所述，它们的转化程度各不相同。将这些细胞系制成悬液，并与一组7种底物接触，即刀豆球蛋白A（Con. A）、纤连蛋白、麦胚凝集素（WGA）、IV型胶原、层粘连蛋白、花生凝集素（PNA）和人工基底膜。使用流式细胞光度法在大量细胞群体上测量细胞黏附，测量样本包含未黏附细胞和作为内标的荧光珠。结果发现，这些细胞系在对所选底物的细胞黏附动力学方面彼此不同。与层粘连蛋白、纤连蛋白或IV型胶原相比，属于已建立细胞系的细胞中，有更高比例的细胞黏附于包被有PNA、WGA或Con A的表面。细胞对纤连蛋白、层粘连蛋白或IV型胶原包被表面黏附的差异使得区分单个细胞系成为可能。对所有细胞系黏附的总体模式进行判别分析以作比较。通过将即时结果与在37℃恢复3小时后的细胞所获结果相比较，评估胰蛋白酶处理对细胞表面结构的影响以及可能伴随的黏附变化。结果发现，所有细胞系在恢复后对层粘连蛋白的黏附性更好。这与已发表的关于层粘连蛋白受体对胰蛋白酶处理高度敏感的数据一致。另一方面，恢复期后PNA、WGA和Con A的受体表达减少，这表现为细胞对这三种底物的黏附性降低。细胞恢复对其纤连蛋白和IV型胶原受体数量的影响在不同细胞系中有所不同，但对于这一对受体，在给定细胞系中变化方向是相同的。在某些情况下，恢复期对细胞黏附没有任何影响。属于已建立细胞系的细胞对所选底物组的不同黏附模式可作为表征单个细胞系的一个参数，但它与它们的转化程度无关。
