Holmberg S B, Hafström L, Jacobsson L
Department of Surgery, Sahlgrenska Hospital, University of Göteborg, Sweden.
Anticancer Res. 1990 Mar-Apr;10(2A):407-9.
This study describes in vivo methodology for evaluating whole body and Tumour Adjacent Macrophage (TAM) phagocytic function in tumour-bearing rats. 29 Wistar rats were inoculated with 1 x 5 mm cylinders of syngeneic NGW-adenocarcinoma im in the right hind leg. 10 rats were given iv zymosan (3mg/100 g b.w.) for systemic activation of macrophages, 9 rats were given intratumour injection of zymosan (3mg/100g b.w.) for local activation and 10 rats were untreated controls. 10 days after tumour inoculation whole body phagocytic rate was estimated with dynamic liver RES scintigraphy. Tumour and blood radionuclide activity was measured. Dynamic liver RES scintigraphy measures whole body macrophage phagocytic rate (k) of iv 99Tcm Nanocoll albumin test colloid. TAM phagocytic rate is calculated from the final uptake in tumor/injected dose x k. Systemic macrophage activation caused a significant increase in liver and spleen weight and whole body phagocytic rate (0.65 +/- 0.02 versus 0.56 +/- 0.03% s-1). The TAM phagocytic rate was significantly elevated in tumors treated by local macrophage activation (0.003 +/- 0.00 versus 0.001 +/- 0.00% s-1). This study suggests that the TAM phagocytic rate is a sensitive indication of local macrophage activity.
本研究描述了在荷瘤大鼠中评估全身及肿瘤相关巨噬细胞（TAM）吞噬功能的体内方法。29只Wistar大鼠右后腿接种1×5mm同基因NGW - 腺癌圆柱体。10只大鼠静脉注射酵母聚糖（3mg/100g体重）以全身激活巨噬细胞，9只大鼠肿瘤内注射酵母聚糖（3mg/100g体重）以局部激活，10只大鼠作为未处理对照。肿瘤接种10天后，用动态肝脏网状内皮系统闪烁扫描法评估全身吞噬率。测量肿瘤和血液中的放射性核素活性。动态肝脏网状内皮系统闪烁扫描法测量静脉注射99Tcm纳米胶体白蛋白试验胶体后的全身巨噬细胞吞噬率（k）。TAM吞噬率由肿瘤最终摄取量/注射剂量×k计算得出。全身巨噬细胞激活导致肝脏和脾脏重量及全身吞噬率显著增加（0.65±0.02对0.56±0.03%s-1）。局部巨噬细胞激活处理的肿瘤中TAM吞噬率显著升高（0.003±0.00对0.001±0.00%s-1）。本研究表明，TAM吞噬率是局部巨噬细胞活性的敏感指标。
