Holmberg S B, Hafström L, Jacobsson L
Department of Surgery, Sahlgrenska Hospital, Göteborg, Sweden.
Anticancer Res. 1988 Nov-Dec;8(6):1291-5.
Dynamic liver RES scintigraphy with Nanocoll (99Tcm albumin colloid - 50 nm diameter) assessing RES-macrophage phagocytic function was performed in 40 control, 73 RES-stimulated non-tumour-bearing and 59 tumour-bearing Wistar/FU rats in vivo. Tumour-bearing rats were inoculated with 10(6) x 1.0 cells of a syngeneic nitrosoguanidine-induced colonic carcinoma in the liver. Twenty-eight of these rats had been treated one day previously with Zymosan (3 mg x 100 g-1 i v) as a RES stimulant. The clearance/uptake rate (k) of Nanocoll was calculated from dynamic liver images by the slope in the plot 1n [1 - U(t)/U] versus t where t is time and U liver uptake. k-value in control animals was 0.45 +/- 0.01.10(-2) x s-1 Zymosan injection in non-tumour-bearing rats caused stati-stically significant higher clearance/uptake rate on day 1, through 8 after treatment compared to that of controls. On day 8 k-value was 0.64 +/- 0.04. In tumour-bearing rats the uptake rate (k) was on day 8 0.66 +/- 0.03, while in RES-stimulated tumour-bearing rats it was 0.64 +/- 0.03. Survival was 22 +/- 1 days in tumour bearing rats and 37 +/- 4 days in RES stimulated tumour-bearing rats. The average tumour volume after one week was 132 +/- 24 mm3 in non-stimulated rats and 20 +/- 5 mm3 in RES stimulated rats. There was a negative correlation between uptake rate and tumour volume and a positive correlation between uptake rate and survival on day 8 in non-stimulated tumour-bearing rats. Dynamic liver RES scintigraphy with small size 99Tcm albumin colloid (Nanocoll) can be used to measure RES phagocytic function and the effect of liver tumour growth on RES.
对40只对照Wistar/FU大鼠、73只经网状内皮系统（RES）刺激的无瘤Wistar/FU大鼠以及59只荷瘤Wistar/FU大鼠进行了使用纳米胶体（直径50纳米的99锝标记白蛋白胶体）的动态肝脏RES闪烁扫描，以评估RES巨噬细胞的吞噬功能。给荷瘤大鼠接种10(6)×1.0个同基因亚硝基胍诱导的肝癌细胞。其中28只大鼠在一天前接受了酵母聚糖（3毫克×100克-1静脉注射）作为RES刺激剂的治疗。通过绘制1n [1 - U(t)/U]与t的关系图（其中t为时间，U为肝脏摄取量），从动态肝脏图像计算纳米胶体的清除/摄取率（k）。对照动物的k值为0.45±0.01×10(-2)×秒-1。在无瘤大鼠中，注射酵母聚糖后第1天至治疗后8天，清除/摄取率在统计学上显著高于对照组。在第8天，k值为0.64±0.04。在荷瘤大鼠中，第8天的摄取率（k）为0.66±0.03，而在经RES刺激的荷瘤大鼠中为0.64±0.03。荷瘤大鼠的生存期为22±1天，经RES刺激的荷瘤大鼠为37±4天。一周后，未受刺激的大鼠平均肿瘤体积为132±24立方毫米，经RES刺激的大鼠为20±5立方毫米。在未受刺激的荷瘤大鼠中，第8天的摄取率与肿瘤体积呈负相关，与生存期呈正相关。使用小尺寸99锝标记白蛋白胶体（纳米胶体）的动态肝脏RES闪烁扫描可用于测量RES吞噬功能以及肝肿瘤生长对RES的影响。
