Anhui Key Laboratory of Chemo-Biosensing, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Analyst. 2013 Apr 21;138(8):2392-7. doi: 10.1039/c3an36921h.
A new luminescence resonant energy transfer (LRET) system has been designed that utilizes near-infrared (NIR)-to-NIR upconversion lanthanide nanophosphors (UCNPs) as the donor, and Au nanorods (Au NRs) as the acceptor. The UCNPs were excited by a near-infrared (980 nm) wavelength and also emitted at a near-infrared wavelength (804 nm) using an inexpensive infrared continuous wave laser diode. The Au NRs showed a high absorption band around 806 nm, which provided large spectral overlap between the donor and the acceptor. Hg(2+) ions were added to an aqueous solution containing the UCNPs and Au NRs that were modified with a Hg(2+) aptamer. Then, a sandwich-type LRET system was developed for the detection of Hg(2+) ions that had high sensitivity and selectivity in the NIR region. The method was successfully applied to the sensing of Hg(2+) ions in water and human serum samples.
一种新的荧光共振能量转移（LRET）系统已经被设计出来，该系统利用近红外（NIR）-近红外上转换镧系纳米荧光粉（UCNP）作为供体，金纳米棒（Au NRs）作为受体。UCNP 被近红外（980nm）波长激发，同时使用廉价的红外连续波激光二极管在近红外波长（804nm）处发射。Au NRs 在 806nm 左右表现出一个高吸收带，这为供体和受体之间提供了较大的光谱重叠。Hg（2+）离子被添加到含有 UCNP 和用 Hg（2+）适配体修饰的 Au NRs 的水溶液中。然后，开发了一种用于检测 Hg（2+）离子的三明治型 LRET 系统，该系统在近红外区域具有高灵敏度和选择性。该方法成功地应用于水和人血清样品中 Hg（2+）离子的传感。
