Mane-Gavade Shubhangi, Patil Supriya A, Jadhav Vidhya, Pattanshetti Akshata, Nipane Sandip, Yu Xiao-Ying, Kim Deok-Kee, Sabale Sandip
Department of Chemistry, Jaysingpur College Jaysingpur (Shivaji University Kolhapur), Jaysingpur, MS, 416101, India.
Department of Nanotechnology and Advanced Materials Engineering, Sejong University, Seoul, 05006, Republic of Korea.
Photochem Photobiol Sci. 2025 Apr;24(4):659-668. doi: 10.1007/s43630-025-00718-7. Epub 2025 Apr 29.
Gold nanoparticles (AuNPs) have gained attention due to their unique optical and electronic properties. Their exceptional stability, high conductivity, and strong light interaction also make them ideal for sensing applications. In this study, we developed a green microwave-assisted approach for synthesizing uniform (~ 10 nm) AuNPs using Acacia concinna fruit extract as a bio-stabilizing agent. Synthesized AuNPs were employed for a rapid and straightforward AuNPs-based sensing probe for the detection of mercury ions (Hg) in aqueous samples and merbromin medicine, exhibiting fluorescence at 793 nm. The sensing probe was established using a fluorescence quenching approach for selective detection of Hg⁺. Results indicate that the AuNPs demonstrate high selectivity for Hg⁺ compared to other cations, including Pb⁺, Cd⁺, Fe⁺, Ni⁺, Ba⁺, Zn⁺, Cu, Ag and Cr. A strong correlation between Hg⁺ concentration and the observed fluorescence intensity ratio (F₀/F) enables precise quantitative detection, with a limit of detection (LOD) of 0.60 µg/mL in water samples. This study highlights the effectiveness of the fluorescence quenching method as a cost-effective, rapid, and simple tool for Hg⁺ detection in both environmental water samples and medicinal applications.
金纳米颗粒（AuNPs）因其独特的光学和电子特性而受到关注。它们出色的稳定性、高导电性和强烈的光相互作用也使其成为传感应用的理想选择。在本研究中，我们开发了一种绿色微波辅助方法，以金合欢果实提取物作为生物稳定剂来合成均匀的（约10纳米）AuNPs。合成的AuNPs被用于构建一种快速且直接的基于AuNPs的传感探针，用于检测水性样品和汞溴红药物中的汞离子（Hg），该探针在793纳米处呈现荧光。该传感探针采用荧光猝灭方法建立，用于选择性检测Hg⁺。结果表明，与其他阳离子（包括Pb⁺、Cd⁺、Fe⁺、Ni⁺、Ba⁺、Zn⁺、Cu、Ag和Cr）相比，AuNPs对Hg⁺表现出高选择性。Hg⁺浓度与观察到的荧光强度比（F₀/F）之间存在强相关性，能够实现精确的定量检测，在水样中的检测限（LOD）为0.60微克/毫升。本研究突出了荧光猝灭方法作为一种经济高效、快速且简单的工具，用于环境水样和药物应用中Hg⁺检测的有效性。
