Chemical Physics, Lund University, Box 124, 22100, Lund, Sweden.
Nanoscale. 2013 Apr 7;5(7):3070-7. doi: 10.1039/c3nr33513e.
We propose to combine the method of fluorescence intensity centroid localization with rotation of the plane of excitation polarization. Polarized light interacts selectively with differently oriented fluorophores; thus yielding topological information on the nanometer scale, without any need for fluorophore blinking. The method is applicable to photostable individual systems, when most of the traditional super-resolution methods fail. A theoretical study is supported by experiments on 30 nm long cyclodextrin-encapsulated single polyrotaxane conjugated polymer chains.
我们提出将荧光强度质心定位方法与激发偏振面的旋转相结合。偏振光与不同取向的荧光团选择性相互作用；因此，在不需要荧光团闪烁的情况下，提供关于纳米级的拓扑信息。当大多数传统的超分辨率方法都失效时，该方法适用于光稳定的单个系统。通过对 30nm 长的环糊精封装的单聚轮烷共轭聚合物链的实验，对该理论进行了研究。
