Advanced Photonics Center, Southeast University, Nanjing, People's Republic of China.
J Fluoresc. 2013 May;23(3):551-9. doi: 10.1007/s10895-013-1187-9. Epub 2013 Mar 5.
A straightforward immunoassay based on surface enhanced fluorescence (SEF) has been demonstrated using a fluorescent immune substrate and antibody functionalized-silver nanoparticles. Unlike the conventional SEF-based immunoassay, which usually uses the dye-labeled antibodies and the metallic nanostructured-substrates, the presented immune system does not need the antibodies to be labeled with dye molecules. Thus, this immunoassay can be easily applied to the detection of a wide range of target antigens, which is of great importance for its practical application. The experimental results show that this immunoassay has a good specificity as well as the capacity of quantitative detection. Basically, the surface density of the immuno-adsorbed silver nanoparticles increases with the increased amount of target antigens, resulting in a fluorescence enhancement up to around 7 fold. The dose-responsive performance of the immunoassay has been investigated and the limit of detection (LOD) is 1 ng/mL. Due to its simple preparation method and the wide range of detectable antigens, this presented immunoassay is expected to be helpful for extending the SEF-based application.
一种基于表面增强荧光（SEF）的简单免疫分析方法已经被证明，该方法使用荧光免疫底物和抗体功能化的银纳米粒子。与传统的基于 SEF 的免疫分析方法不同，后者通常使用染料标记的抗体和金属纳米结构底物，本免疫体系不需要将抗体标记上染料分子。因此，这种免疫分析方法可以很容易地应用于广泛的目标抗原的检测，这对于其实际应用非常重要。实验结果表明，该免疫分析具有良好的特异性和定量检测能力。基本上，免疫吸附的银纳米粒子的表面密度随着目标抗原的增加而增加，导致荧光增强高达约 7 倍。已经研究了免疫分析的剂量响应性能，检测限（LOD）为 1ng/mL。由于其简单的制备方法和广泛的可检测抗原，本免疫分析有望有助于扩展基于 SEF 的应用。
