Romanian Academy, Institute of Physical Chemistry Ilie Murgulescu, Bucharest, Romania.
J Fluoresc. 2013 May;23(3):569-74. doi: 10.1007/s10895-013-1204-z. Epub 2013 Mar 6.
The photophysical properties of luminol in a silver nanoparticles complex have been studied by steady-state and time resolved fluorescence spectroscopy. The effect of the serum albumin on the luminol fluorescence in the silver nanoparticles has been also investigated. It was found that the fluorescence quantum yield value of luminol in a silver nanoparticles complex is φ = 0.00407. The decrease of the average fluorescence lifetime value of the luminol in the silver nanoparticles complex was found to be low, <τ> = 1.712 ns. The luminol does not bind to the serum albumins in the presence of silver nanoparticles. The formation of a new species of luminol on silver nanoparticles is discussed. The results have influence regarding the use of luminol as an assay for bio-analytical applications.
通过稳态和时间分辨荧光光谱法研究了银纳米粒子配合物中鲁米诺的光物理性质。还研究了血清白蛋白对银纳米粒子中鲁米诺荧光的影响。结果发现，银纳米粒子配合物中鲁米诺的荧光量子产率值φ=0.00407。发现银纳米粒子配合物中鲁米诺的平均荧光寿命值<τ>降低较小，为 1.712 ns。在银纳米粒子存在的情况下，鲁米诺不与血清白蛋白结合。讨论了在银纳米粒子上形成鲁米诺新物种的问题。这些结果对鲁米诺作为生物分析应用的测定方法的使用有影响。
