Szmacinski Henryk, Ray Krishanu, Lakowicz Joseph R
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, Baltimore, MD 21201, USA.
Anal Biochem. 2009 Feb 15;385(2):358-64. doi: 10.1016/j.ab.2008.11.025. Epub 2008 Nov 27.
The detection of submonolayers of proteins based on native fluorescence is a potentially valuable approach for label-free detection. We have examined the possibility of using silver nanostructures to increase the emission of tryptophan residues in proteins. Fluorescence spectra, intensities, and lifetimes of multilayers and submonolayers of proteins deposited on the surfaces of silver island films were measured. Increased fluorescence intensities from two- to three-fold and similar decreases in lifetimes were observed in the presence of the silver nanoparticles compared with the proteins on the surface of the bare quartz. The observed spectral effects of silver nanoparticles on tryptophan fluorescence indicates the possibility for the design of analytical tools for the detection of proteins without traditional labeling by extrinsic fluorophores.
基于天然荧光检测蛋白质亚单层是一种具有潜在价值的无标记检测方法。我们研究了使用银纳米结构增强蛋白质中色氨酸残基发射的可能性。测量了沉积在银岛膜表面的蛋白质多层膜和亚单层膜的荧光光谱、强度和寿命。与裸石英表面的蛋白质相比，在存在银纳米颗粒的情况下，观察到荧光强度增加了两到三倍，寿命也有类似程度的降低。银纳米颗粒对色氨酸荧光的光谱效应表明，有可能设计出无需传统外在荧光团标记即可检测蛋白质的分析工具。
