蛋白质中色氨酸残基的金属增强荧光：在无标记生物测定中的应用。
Metal-enhanced fluorescence of tryptophan residues in proteins: application toward label-free bioassays.
作者信息
Szmacinski Henryk, Ray Krishanu, Lakowicz Joseph R
机构信息
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, Baltimore, MD 21201, USA.
出版信息
Anal Biochem. 2009 Feb 15;385(2):358-64. doi: 10.1016/j.ab.2008.11.025. Epub 2008 Nov 27.
Abstract
The detection of submonolayers of proteins based on native fluorescence is a potentially valuable approach for label-free detection. We have examined the possibility of using silver nanostructures to increase the emission of tryptophan residues in proteins. Fluorescence spectra, intensities, and lifetimes of multilayers and submonolayers of proteins deposited on the surfaces of silver island films were measured. Increased fluorescence intensities from two- to three-fold and similar decreases in lifetimes were observed in the presence of the silver nanoparticles compared with the proteins on the surface of the bare quartz. The observed spectral effects of silver nanoparticles on tryptophan fluorescence indicates the possibility for the design of analytical tools for the detection of proteins without traditional labeling by extrinsic fluorophores.
摘要
基于天然荧光检测蛋白质亚单层是一种具有潜在价值的无标记检测方法。我们研究了使用银纳米结构增强蛋白质中色氨酸残基发射的可能性。测量了沉积在银岛膜表面的蛋白质多层膜和亚单层膜的荧光光谱、强度和寿命。与裸石英表面的蛋白质相比，在存在银纳米颗粒的情况下，观察到荧光强度增加了两到三倍，寿命也有类似程度的降低。银纳米颗粒对色氨酸荧光的光谱效应表明，有可能设计出无需传统外在荧光团标记即可检测蛋白质的分析工具。
相似文献
1
Metal-enhanced fluorescence of tryptophan residues in proteins: application toward label-free bioassays.蛋白质中色氨酸残基的金属增强荧光：在无标记生物测定中的应用。
Anal Biochem. 2009 Feb 15;385(2):358-64. doi: 10.1016/j.ab.2008.11.025. Epub 2008 Nov 27.
2
Enhanced fluorescence of proteins and label-free bioassays using aluminum nanostructures.利用铝纳米结构增强蛋白质的荧光和无标记生物分析。
Anal Chem. 2009 Aug 1;81(15):6049-54. doi: 10.1021/ac900263k.
3
Metal-enhanced Intrinsic Fluorescence of Proteins on Silver Nanostructured Surfaces towards Label-Free Detection.银纳米结构表面上蛋白质的金属增强固有荧光用于无标记检测
J Phys Chem C Nanomater Interfaces. 2008;112(46):17957-17963. doi: 10.1021/jp807025n.
4
Radiative decay engineering. 2. Effects of Silver Island films on fluorescence intensity, lifetimes, and resonance energy transfer.辐射衰变工程。2. 银岛膜对荧光强度、寿命和共振能量转移的影响。
Anal Biochem. 2002 Feb 15;301(2):261-77. doi: 10.1006/abio.2001.5503.
5
Metal Enhanced Intrinsic Fluorescence of Proteins and Label-Free Bioassays.蛋白质的金属增强固有荧光及无标记生物测定
Proc SPIE Int Soc Opt Eng. 2010 Mar 1;7577:75770K. doi: 10.1117/12.840412.
6
Nonlinear excitation of tryptophan emission enhanced by silver nanoparticles.银纳米颗粒增强色氨酸发射的非线性激发
J Fluoresc. 2008 Nov;18(6):1151-5. doi: 10.1007/s10895-008-0366-6. Epub 2008 Apr 1.
7
Fast and slow deposition of silver nanorods on planar surfaces: application to metal-enhanced fluorescence.银纳米棒在平面表面上的快速和慢速沉积：在金属增强荧光中的应用。
J Phys Chem B. 2005 Mar 3;109(8):3157-62. doi: 10.1021/jp045186t.
8
A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution.用于单细菌追踪的荧光纳米探针：用色氨酸对银纳米颗粒进行功能化以实现单细胞分辨率下纳米颗粒积累的探测。
Analyst. 2016 Mar 21;141(6):1988-96. doi: 10.1039/c5an02358k.
9
Study of the tryptophan-terbium FRET pair coupled to silver nanoprisms for biosensing applications.用于生物传感应用的色氨酸-铽能量转移对与银纳米棱柱耦合的研究。
Phys Chem Chem Phys. 2013 Jun 14;15(22):8838-44. doi: 10.1039/c3cp50742d. Epub 2013 May 3.
10
Fluorescence enhancements on silver colloid coated surfaces.银胶体涂层表面的荧光增强。
J Fluoresc. 2004 Jul;14(4):417-23. doi: 10.1023/b:jofl.0000031823.04926.75.
引用本文的文献
1
Plasmonic Fluorescence Enhancement in Diagnostics for Clinical Tests at Point-of-Care: A Review of Recent Technologies.即时医疗临床检测诊断中的表面等离子体激元荧光增强：近期技术综述
Adv Mater. 2023 Aug;35(34):e2107986. doi: 10.1002/adma.202107986. Epub 2022 Apr 24.
2
Review: Applications of surface-enhanced fluorescence (SEF) spectroscopy in bio-detection and biosensing.综述：表面增强荧光（SEF）光谱在生物检测与生物传感中的应用。
Sens Biosensing Res. 2020 Dec;30:100382. doi: 10.1016/j.sbsr.2020.100382. Epub 2020 Oct 16.
3
Alpinia calcarata: potential source for the fabrication of bioactive silver nanoparticles.距花山姜：制备生物活性银纳米颗粒的潜在来源。
Nano Converg. 2018 Dec 6;5(1):37. doi: 10.1186/s40580-018-0167-9.
4
Association of breast carcinoma growth with a non-canonical axis of IFNγ/IDO1/TSP1.乳腺癌生长与IFNγ/IDO1/TSP1非经典轴的关联
Oncotarget. 2017 Jun 28;8(49):85024-85039. doi: 10.18632/oncotarget.18781. eCollection 2017 Oct 17.
5
Interaction of gallic acid with trypsin analyzed by spectroscopy.通过光谱法分析没食子酸与胰蛋白酶的相互作用。
J Food Drug Anal. 2015 Jun;23(2):234-242. doi: 10.1016/j.jfda.2014.09.001. Epub 2014 Dec 4.
6
Synthesis of Fluorescent Core-Shell Metal Nanohybrids: A Versatile Approach.荧光核壳金属纳米杂化物的合成：一种通用方法。
Materials (Basel). 2016 Dec 9;9(12):997. doi: 10.3390/ma9120997.
7
Metal-Enhanced Fluorescence from Silver Nanowires with High Aspect Ratio on Glass Slides for Biosensing Applications.用于生物传感应用的载玻片上具有高纵横比的银纳米线的金属增强荧光
J Phys Chem C Nanomater Interfaces. 2015 Jan 8;119(1):675-684. doi: 10.1021/jp509040f. Epub 2014 Dec 10.
8
Fluoxetine competes with cortisol for binding to human serum albumin.氟西汀与皮质醇竞争结合人血清白蛋白。
Iran J Pharm Res. 2012 Winter;11(1):325-30.
9
On the fluorescence of luminol in a silver nanoparticles complex.鲁米诺在银纳米粒子配合物中的荧光。
J Fluoresc. 2013 May;23(3):569-74. doi: 10.1007/s10895-013-1204-z. Epub 2013 Mar 6.
10
Largely enhanced single-molecule fluorescence in plasmonic nanogaps formed by hybrid silver nanostructures.通过杂交银纳米结构形成的等离子体纳米间隙中，单分子荧光得到了极大增强。
Langmuir. 2013 Feb 26;29(8):2731-8. doi: 10.1021/la3048399. Epub 2013 Feb 14.
本文引用的文献
1
An Overview of Label-free Electrochemical Protein Sensors.无标记电化学蛋白质传感器概述
Sensors (Basel). 2007 Dec 20;7(12):3442-3458. doi: 10.3390/s7123442.
2
The fluorescence enhancement of the protein adsorbed on the surface of Ag nanoparticle.吸附在银纳米颗粒表面的蛋白质的荧光增强。
J Fluoresc. 2009 Jan;19(1):111-7. doi: 10.1007/s10895-008-0392-4. Epub 2008 Jul 15.
3
Aluminum nanostructured films as substrates for enhanced fluorescence in the ultraviolet-blue spectral region.铝纳米结构薄膜作为用于增强紫外-蓝光光谱区域荧光的基底。
Anal Chem. 2007 Sep 1;79(17):6480-7. doi: 10.1021/ac071363l. Epub 2007 Aug 8.
4
Label-free, single-molecule detection with optical microcavities.利用光学微腔进行无标记单分子检测。
Science. 2007 Aug 10;317(5839):783-7. doi: 10.1126/science.1145002. Epub 2007 Jul 5.
5
Nucleic acid and protein mass mapping by live-cell deep-ultraviolet microscopy.通过活细胞深紫外显微镜进行核酸和蛋白质质量图谱分析。
Nat Methods. 2007 Jul;4(7):567-9. doi: 10.1038/nmeth1053. Epub 2007 Jun 3.
6
Fabrication of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species.用于生物和化学物质超灵敏、无标记、实时检测的硅纳米线器件的制造。
Nat Protoc. 2006;1(4):1711-24. doi: 10.1038/nprot.2006.227.
7
Surface-enhanced Raman scattering based nonfluorescent probe for multiplex DNA detection.基于表面增强拉曼散射的用于多重DNA检测的非荧光探针。
Anal Chem. 2007 Jun 1;79(11):3981-8. doi: 10.1021/ac070078z. Epub 2007 Apr 28.
8
Combined enhanced fluorescence and label-free biomolecular detection with a photonic crystal surface.利用光子晶体表面实现荧光增强与无标记生物分子检测相结合
Appl Opt. 2007 Apr 20;46(12):2351-60. doi: 10.1364/ao.46.002351.
9
Recent advances in surface plasmon resonance based techniques for bioanalysis.基于表面等离子体共振的生物分析技术的最新进展。
Anal Bioanal Chem. 2007 Mar;387(5):1831-40. doi: 10.1007/s00216-006-1052-7. Epub 2007 Jan 4.
10
Non-optical screening platforms: the next wave in label-free screening?非光学筛选平台：无标记筛选的下一波浪潮？
Drug Discov Today. 2006 Dec;11(23-24):1068-74. doi: 10.1016/j.drudis.2006.10.001. Epub 2006 Oct 20.
Zotero 插件