Billiar T R, Curran R D, Stuehr D J, Stadler J, Simmons R L, Murray S A
Department of Neurobiology, Anatomy, and Cell Science, University of Pittsburgh, PA 15261.
Biochem Biophys Res Commun. 1990 May 16;168(3):1034-40. doi: 10.1016/0006-291x(90)91133-d.
The in vivo conditions needed for the induction of nitrogen oxide synthesis by hepatocytes were determined. Hepatocytes obtained from rats injected with killed Corynebacterium parvum spontaneously produced NO2(-)+NO3- in culture and were found to contain cytosolic enzyme activity for nitrogen oxide synthesis. The enzyme activity required both L-arginine and NADPH, and was not found in hepatocytes obtained from normal rats or rats injected with lipopolysaccharide (LPS) alone. In contrast, nonparenchymal cells were stimulated to synthesize NO2(-)+NO3- by LPS. These results show the presence of inducible cytosolic enzyme activity for nitrogen oxide synthesis in hepatocytes, which is distinct from nonparenchymal cell NO. synthesis.
确定了肝细胞诱导合成氮氧化物所需的体内条件。从注射了灭活微小棒状杆菌的大鼠获得的肝细胞在培养中自发产生NO2(-)+NO3-，并且发现其含有用于氮氧化物合成的胞质酶活性。该酶活性需要L-精氨酸和NADPH，而在从正常大鼠或仅注射脂多糖（LPS）的大鼠获得的肝细胞中未发现。相反，LPS刺激非实质细胞合成NO2(-)+NO3-。这些结果表明肝细胞中存在可诱导的用于氮氧化物合成的胞质酶活性，这与非实质细胞的NO合成不同。
