Department of Oncology, Zhongnan Hospital of Wuhan University, No 169 Donghu Road, Wuchang District, Wuhan 430071, PR China.
Biomaterials. 2013 May;34(15):3816-27. doi: 10.1016/j.biomaterials.2013.02.018. Epub 2013 Mar 7.
Metastatic recurrence is the most important biological behavior of hepatocellular carcinoma (HCC) and the main cause of treatment failure. Early prediction of metastasis is currently impossible due to the lack of specific molecular probes to recognize metastatic HCC cells. Aptamers have recently emerged as promising potential molecular probes for biomedical applications. Two well-matched HCC cell lines including HCCLM9 with high metastatic potential and MHCC97-L with low metastatic potential, were used to select aptamers for HCC metastasis. With a whole-cell-SELEX strategy, in which HCCLM9 cells were used as target cells and MHCC97-L cells as subtractive cell, 6 potential aptamers had been generated. Detailed study on selected aptamer LY-1 revealed that it could bind metastatic HCC cells with high affinity and specificity, not only in cells culture and animal models of HCC metastasis, but also in clinical HCC specimens. Moreover, the aptamer LY-1 and magnetic particles conjugates could efficiently capture the HCC cells from complex mixture whole blood. These studies demonstrated that this HCC specific aptamer LY-1 could be a promising molecular probe to recognize metastatic HCC cells.
转移复发是肝细胞癌（HCC）最重要的生物学行为，也是治疗失败的主要原因。由于缺乏识别转移性 HCC 细胞的特异性分子探针，目前无法早期预测转移。适体作为生物医学应用中很有前途的潜在分子探针最近出现。使用全细胞 SELEX 策略，将 HCCLM9 细胞用作靶细胞，将 MHCC97-L 细胞用作消减细胞，选择用于 HCC 转移的适体。选择的适体 LY-1 可以与高转移性 HCC 细胞高度结合，特异性高，不仅在 HCC 转移的细胞培养和动物模型中，而且在临床 HCC 标本中也是如此。此外，适体 LY-1 和磁性颗粒缀合物可以有效地从复杂的混合全血中捕获 HCC 细胞。这些研究表明，这种 HCC 特异性适体 LY-1 可以成为识别转移性 HCC 细胞的有前途的分子探针。
