印度北部血管紧张素转换酶（ACE）和脂肪酸结合蛋白2（FABP2）基因多态性与2型糖尿病的关联

Association of angiotensin-converting enzyme (ACE) and fatty acid binding protein 2 (FABP2) genes polymorphism with type 2 diabetes mellitus in Northern India.

作者信息

Raza Syed Tasleem, Fatima Jalees, Ahmed Faisal, Abbas Shania, Zaidi Zeashan Haider, Singh Seema, Mahdi Farzana

机构信息

Department of Biochemistry, Era's Lucknow Medical College and Hospital, India

Department of Medicine, Era's Lucknow Medical College and Hospital, India.

出版信息

J Renin Angiotensin Aldosterone Syst. 2014 Dec;15(4):572-9. doi: 10.1177/1470320313481082. Epub 2013 Mar 6.

DOI:10.1177/1470320313481082

PMID:23468166

Abstract

BACKGROUND

Type 2 diabetes mellitus (T2DM) is growing in an epidemic manner across the world with an expected doubling of the incidence to millions of affected individuals in the last decades. At present, adequate data are not available regarding the ACE and FABP2 polymorphisms and their susceptibility with T2DM cases in the North Indian population. Thus we conceived the need for further study of ACE (I/D) and FABP2 (Ala54Thr) genes polymorphism and its susceptibility to T2DM in the North Indian population.

MATERIALS AND METHODS

In this study, a total of 300 subjects (including 190 T2DM cases and 110 controls) participated. ACE and FABP2 gene polymorphisms in the cases and controls were evaluated by polymerase chain reaction and restriction fragment length polymorphism.

RESULTS

The frequencies of ACE I/I, I/D and D/D genotypes in T2DM cases and controls were 28.73%, 55.17%, 16.09% and 13.63%, 57.95%, 28.40%, respectively. The frequencies of FABP2 Ala54Ala, Ala54Thr and Thr54Thr in T2DM cases were 18.39%, 66.66%, 14.94% and 22.72%, 61.36%, 15.90% in controls, respectively. ACE I/I genotype was significantly more frequent in cases as compared to controls (p = 0.003, χ(2) = 9.13).

CONCLUSION

It appears that the ACE I/I genotype frequency was significantly higher in the T2DM cases as compared to the controls.

摘要

背景

2型糖尿病（T2DM）在全球正呈流行态势增长，预计在过去几十年中发病率将翻倍，受影响个体达数百万。目前，关于北印度人群中血管紧张素转换酶（ACE）和脂肪酸结合蛋白2（FABP2）基因多态性及其与T2DM易感性的充分数据尚不具备。因此，我们认为有必要进一步研究北印度人群中ACE（I/D）和FABP2（Ala54Thr）基因多态性及其对T2DM的易感性。

材料与方法

本研究共纳入300名受试者（包括190例T2DM患者和110名对照）。通过聚合酶链反应和限制性片段长度多态性评估病例组和对照组中ACE和FABP2基因多态性。

结果

T2DM病例组和对照组中ACE I/I、I/D和D/D基因型频率分别为28.73%、55.17%、16.09%和13.63%、57.95%、28.40%。T2DM病例组中FABP2 Ala54Ala、Ala54Thr和Thr54Thr基因型频率分别为18.39%、66.66%、14.94%，对照组分别为22.72%、61.36%、15.90%。与对照组相比，病例组中ACE I/I基因型频率显著更高（p = 0.003，χ(2)= 9.13）。