Iwase K, Fujii Y, Nakashima I, Kato N, Fujino Y, Kawashima H, Mochizuki M
Department of Ophthalmology, School of Medicine, University of Tokyo, Japan.
Curr Eye Res. 1990 Mar;9(3):207-16. doi: 10.3109/02713689009044515.
Experimental autoimmune uveoretinitis (EAU) was induced in two strains of mice by repeated-immunization protocol. SMA mice (H-2 nondefined) and C57BL/6 mice (H-2b) were immunized with S-antigen mixed with Klebsiella 03 lipopolysaccharide (K03 LPS) repeatedly at intervals of 1 to 4 weeks. Following the tertiary immunization, the mice exhibited histopathological changes of EAU as well as significant immune responses to the antigen. The antigen doses required for successful EAU induction were 4 micrograms or more at each immunization time. The histopathology of EAU was characterized by mild infiltration of mononuclear cells in the retina and the choroid, particularly, at the retinal blood vessels and the photoreceptor cell layer. The anterior segment of the eye was not affected by inflammation, and therefore clinical signs of EAU were not detected even under an operating microscope. Since the mouse is a genetically and immunologically well-defined species, this model is useful for study of immunopathogenic mechanisms of EAU.
通过重复免疫方案在两种小鼠品系中诱导实验性自身免疫性葡萄膜视网膜炎（EAU）。SMA小鼠（H-2未定义）和C57BL/6小鼠（H-2b）每隔1至4周用与肺炎克雷伯菌03脂多糖（K03 LPS）混合的S抗原重复免疫。第三次免疫后，小鼠表现出EAU的组织病理学变化以及对抗原的显著免疫反应。每次免疫成功诱导EAU所需的抗原剂量为4微克或更多。EAU的组织病理学特征是视网膜和脉络膜，特别是视网膜血管和光感受器细胞层有轻度单核细胞浸润。眼前段未受炎症影响，因此即使在手术显微镜下也未检测到EAU的临床体征。由于小鼠是遗传和免疫定义明确的物种，该模型可用于研究EAU的免疫致病机制。
