Stone R A, Wilson C M, Glembotski C C
Department of Ophthalmology, University of Pennsylvania School of Medicine, Philadelphia 19104.
Curr Eye Res. 1990 Mar;9(3):287-91. doi: 10.3109/02713689009044525.
Acid extracts of guinea pig and rhesus monkey anterior uvea, choroid and retina contain immunoreactive VIP. By reversed phase high performance liquid chromatography, the immunoreactive material from these ocular tissues elutes at a position similar to synthetic porcine VIP. Only in the guinea pig anterior uvea is a second smaller peak detected. By size exclusion high performance liquid chromatography, the peptide in the monkey anterior uvea, choroid and retina elutes in the identical position as the synthetic porcine VIP standard with an apparent molecular weight of 3450 daltons. We conclude that a single form of VIP, chromatographically similar to the porcine standard, is the predominant form of the peptide in the eye of guinea pig and rhesus monkey.
豚鼠和恒河猴眼前葡萄膜、脉络膜及视网膜的酸性提取物中含有免疫反应性血管活性肠肽（VIP）。通过反相高效液相色谱法，这些眼组织中的免疫反应性物质在与合成猪VIP相似的位置洗脱。仅在豚鼠眼前葡萄膜中检测到第二个较小的峰。通过尺寸排阻高效液相色谱法，猴眼前葡萄膜、脉络膜及视网膜中的肽在与合成猪VIP标准品相同的位置洗脱，表观分子量为3450道尔顿。我们得出结论，在豚鼠和恒河猴眼中，一种色谱行为与猪标准品相似的单一形式的VIP是该肽的主要形式。
