RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
Cell Stem Cell. 2013 Mar 7;12(3):293-7. doi: 10.1016/j.stem.2013.01.005.
Previous studies of serial cloning in animals showed a decrease in efficiency over repeated iterations and a failure in all species after a few generations. This limitation led to the suggestion that repeated recloning might be inherently impossible because of the accumulation of lethal genetic or epigenetic abnormalities. However, we have now succeeded in carrying out repeated recloning in the mouse through a somatic cell nuclear transfer method that includes a histone deacetylase inhibitor. The cloning efficiency did not decrease over 25 generations, and, to date, we have obtained more than 500 viable offspring from a single original donor mouse. The reprogramming efficiency also did not increase over repeated rounds of nuclear transfer, and we did not see the accumulation of reprogramming errors or clone-specific abnormalities. Therefore, our results show that repeated iterative recloning is possible and suggest that, with adequately efficient techniques, it may be possible to reclone animals indefinitely.
先前关于动物连续克隆的研究表明，在经过多次迭代后，效率会逐渐降低，而且在几代之后，所有物种都会失败。这一限制导致人们提出，由于致命遗传或表观遗传异常的积累，重复克隆可能从根本上是不可能的。然而，我们现在已经通过一种包括组蛋白去乙酰化酶抑制剂的体细胞核转移方法成功地在小鼠中进行了重复克隆。克隆效率在 25 代内没有下降，迄今为止，我们已经从一只原始供体小鼠中获得了 500 多个存活后代。重编程效率在多次核转移中也没有增加，我们没有看到重编程错误或克隆特异性异常的积累。因此，我们的结果表明，重复迭代克隆是可行的，并表明，通过足够高效的技术，可能可以无限期地对动物进行克隆。
