人和小鼠胞质环氧化物水解酶：物理和结构特性
Human and murine cytosolic epoxide hydrolase: physical and structural properties.
作者信息
Dietze E C, Magdalou J, Hammock B D
机构信息
Department of Entomology, University of California, Davis 95616.
出版信息
Int J Biochem. 1990;22(5):461-70. doi: 10.1016/0020-711x(90)90258-5.
PMID:2347424
Abstract
- Human and murine liver cytosolic epoxide hydrolase (CEH) had an apparent Mw of 59,000 by SDS-PAGE. 2. Peptide maps of CNBr, trypsin and Staphylococcus aureus V8 digests, as well as amino acid analysis, showed that human and murine CEH were similar. Uninduced and clofibrate induced murine CEH appeared qualitatively identical. 3. The CEHs shared antigenic determinants as determined by Western blotting. 4. Circular dichroism spectra indicate that human CEH had 39% alpha-helix. Uninduced and clofibrate induced murine CEH had 38 and 35% alpha-helix, respectively.
摘要
- 通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）测定，人和小鼠肝脏胞质环氧化物水解酶（CEH）的表观分子量为59,000。2. 溴化氰（CNBr）、胰蛋白酶和金黄色葡萄球菌V8蛋白酶消化产物的肽图谱以及氨基酸分析表明，人和小鼠的CEH相似。未诱导和经氯贝丁酯诱导的小鼠CEH在质量上看起来相同。3. 经蛋白质印迹法测定，CEH共享抗原决定簇。4. 圆二色光谱表明，人CEH含有39%的α-螺旋。未诱导和经氯贝丁酯诱导的小鼠CEH分别含有38%和35%的α-螺旋。
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