Department of Biological Sciences, Feira de Santana State University, Feira de Santana, Bahia, Brazil.
Mutat Res. 2013 May 15;753(2):72-5. doi: 10.1016/j.mrgentox.2013.02.003. Epub 2013 Mar 7.
The indiscriminate use of anabolic androgenic steroids (AAS) has motivated researchers to investigate the mutagenic action of these substances. The present study, using the mouse bone marrow micronucleus test, evaluates the genotoxic potential of testosterone cypionate (deposteron). Male Swiss mice received intramuscular injections of deposteron at three doses. The animals were sacrificed 24, 48, or 72h after treatment and bone marrow was removed immediately, followed by scoring to count the micronuclei in 2000 polychromatic erythrocytes (PCE). Two hundred erythrocytes/animal were analyzed to determine the PCE-NCE (normochromatic erythrocyte) relationship and to determine the cytotoxic effects. The animals treated with deposteron at the highest dose presented greater numbers of micronuclei. The highest dose caused a decrease in the PCE/NCE relationship, indicating a cytotoxic effect. We conclude that deposteron is genotoxic and cytotoxic in mice.
雄激素（AAS）的滥用促使研究人员研究这些物质的诱变作用。本研究采用小鼠骨髓微核试验，评估了庚酸睾酮（deposteron）的遗传毒性潜力。雄性瑞士小鼠接受庚酸睾酮肌肉注射，剂量分别为 3 个。动物在治疗后 24、48 或 72 小时处死，立即取出骨髓，然后在 2000 个多染红细胞（PCE）中计算微核。对每只动物的 200 个红细胞进行分析，以确定 PCE-NCE（正常染色红细胞）的关系，并确定细胞毒性作用。接受最高剂量 deposteron 治疗的动物表现出更多的微核。最高剂量导致 PCE/NCE 关系下降，表明存在细胞毒性作用。我们得出结论，deposteron 在小鼠中具有遗传毒性和细胞毒性。
