用于基因拔河（gTOW）方法的芯片上并行实时聚合酶链反应

Parallel real-time PCR on a chip for genetic tug-of-war (gTOW) method.

作者信息

Naito Toyohiro, Yatsuhashi Ai, Kaji Noritada, Ando Taeko, Sato Kazuo, Moriya Hisao, Kitano Hiroaki, Yasui Takao, Tokeshi Manabu, Baba Yoshinobu

机构信息

Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan.

出版信息

Anal Sci. 2013;29(3):367-71. doi: 10.2116/analsci.29.367.

DOI:10.2116/analsci.29.367

PMID:23474728

Abstract

A microchip-based real-time polymerase chain reaction (PCR) device has been developed for the genetic tug-of-war (gTOW) method that provides quantitative data for research on biorobustness and systems biology. The device was constructed of a silicon glass chip, a temperature controlling Peltier element, and a microscope. A parallel real-time amplification process of target genes on the plasmids and the housekeeping genes in a model eukaryote Saccharomyces cerevisiae were detected simultaneously, and the copy number of the target genes were estimated. The device provides unique quantitative data that can be used to augment understanding of the system-level properties of living cells.

摘要

一种基于微芯片的实时聚合酶链反应（PCR）装置已被开发用于基因拔河（gTOW）方法，该方法可为生物稳健性和系统生物学研究提供定量数据。该装置由硅玻璃芯片、控温珀尔帖元件和显微镜组成。同时检测了模式真核生物酿酒酵母中质粒上目标基因和管家基因的平行实时扩增过程，并估算了目标基因的拷贝数。该装置提供了独特的定量数据，可用于增强对活细胞系统水平特性的理解。