STMicroelectronics, Stradale primosole, Catania.
Distretto Tecnologico Sicilia Micro e Nano Sistemi, VIII Strada, 5-Zona Industriale, Catania, Italy.
Biotechnol Bioeng. 2019 Aug;116(8):2087-2094. doi: 10.1002/bit.26987. Epub 2019 Apr 20.
In this paper, a miniaturized biosensor containing 96 silicon microchambers electroloaded with nano-volumes of liquid (EW-chip) is presented. The liquid electroloading is achieved by the appropriate modulation of interface properties. The surface chemistries have been studied to guarantee effective interface properties for both electrowetting on dielectric actuation and biocompatibility versus biochemical reactions. The silicon microchambers are 200 nl in volume and are connected to a specific system of electrodes able to deliver liquid sample on each well. The device also integrates temperature sensors and heaters to perform biochemical reactions. On that, the effectiveness of this device has been successfully proven towards the nucleic acids detection via real-time polymerase chain reaction amplification. Hepatitis B virus genome target has been used to assess the device performance. Results show very uniform amplification over the 96 microchambers without any cross-contamination process. These features make this system a very appealing potential solution for genetic point-of-care devices where a high level of parallelism of analysis is required.
本文提出了一种包含 96 个硅微室的微型生物传感器，这些硅微室通过电加载纳米体积的液体（EW-chip）。通过适当调节界面特性来实现液体的电加载。研究了表面化学，以保证电润湿介电致动和生物兼容性与生化反应之间的有效界面特性。硅微室的体积为 200nl，并与一个特殊的电极系统相连，该系统能够将液体样本输送到每个微室。该装置还集成了温度传感器和加热器，以进行生化反应。在这方面，该设备已成功用于通过实时聚合酶链反应扩增来检测核酸。乙型肝炎病毒基因组靶标被用于评估该设备的性能。结果表明，在没有任何交叉污染过程的情况下，96 个微室的扩增非常均匀。这些特性使得该系统成为基因即时检测设备的一个非常有吸引力的潜在解决方案，因为这些设备需要高度的分析并行性。
