Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taiwan, ROC.
Agricultural Biotechnology Center, National Chung Hsing University, Taiwan, ROC.
Int J Syst Evol Microbiol. 2013 Sep;63(Pt 9):3293-3300. doi: 10.1099/ijs.0.048579-0. Epub 2013 Mar 8.
A novel Gram-reaction-negative, yellow-pigmented, aerobic, non-motile and rod-shaped bacterium designated strain CC-YHH031(T) was isolated from an agricultural soil collected at Chiayi County, Taiwan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CC-YHH031(T) formed a discrete monophyletic lineage in the family Xanthomonadaceae, sharing high pairwise sequence similarity of 93.5-95.2 and 94.8% with species of the genus Dokdonella (94.9% similarity to the type strain of the type species) and Aquimonas voraii GPTSA 20(T), respectively. The genomic DNA G+C content of strain CC-YHH031(T) was 68.6 ± 0.7 mol% and the predominant respiratory quinone was ubiquinone Q-8. Spermidine was the principal polyamine, with minor amounts of putrescine. Major fatty acids (>5% of total fatty acids) were iso-C(16:00, iso-C(15:0), C(16:1)ω7c and/or C(16:1)ω6c (summed feature 3), iso-C(17:1)ω9c, iso-C(14:0), iso-C(11:0) and iso-C(11:0) 3-OH. The polar lipid profile of strain CC-YHH031(T) included phosphatidylethanolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminophospholipids (APL1-2) and four unidentified phospholipids (PL1-4). Strain CC-YHH031(T) was distinguished particularly from the type species of the genus Dokdonella (Dokdonella koreensis) by the presence of major amounts of iso-C(14:0) and summed feature 3 and minor amounts of iso-C(17:0) and by the complete absence of anteiso-C(17:0), the presence of PL1-3 and APL1-2, the absence of APL3 and the presence of putrescine in the former. On the basis of distinguishing genotypic and phenotypic evidence, strain CC-YHH031(T) is proposed to represent a novel genus and species within the family Xanthomonadaceae, for which the name Chiayiivirga flava gen. nov., sp. nov. is proposed. The type strain of Chiayiivirga flava is CC-YHH031(T) ( =BCRC 80274(T) =DSM 24163(T)).
一株新的革兰氏反应阴性、黄色、需氧、非运动、杆状细菌菌株 CC-YHH031(T) 从台湾嘉义县采集的农业土壤中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CC-YHH031(T) 在黄单胞菌科中形成了一个离散的单系，与 Dokdonella 属的种（与模式种的相似度为 94.9%）和 Aquimonas voraii GPTSA 20(T) 的相似度分别为 93.5-95.2%和 94.8%。菌株 CC-YHH031(T) 的基因组 DNA G+C 含量为 68.6±0.7mol%，主要呼吸醌为泛醌 Q-8。腐胺是主要的多胺，少量精胺。主要脂肪酸（占总脂肪酸的 5%以上）为 iso-C(16:00、iso-C(15:0)、C(16:1)ω7c 和/或 C(16:1)ω6c（特征 3 总和）、iso-C(17:1)ω9c、iso-C(14:0)、iso-C(11:0) 和 iso-C(11:0) 3-OH。菌株 CC-YHH031(T) 的极性脂谱包括磷脂酰乙醇胺、单甲基磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油、两种未鉴定的氨基磷脂（APL1-2）和四种未鉴定的磷脂（PL1-4）。菌株 CC-YHH031(T) 与 Dokdonella 属的模式种（Dokdonella koreensis）的区别特别在于存在大量的 iso-C(14:0)和特征 3，以及少量的 iso-C(17:0)，并且完全不存在 anteiso-C(17:0)、PL1-3 和 APL1-2 的存在、APL3 的不存在以及腐胺的存在。基于区分基因型和表型证据，菌株 CC-YHH031(T) 被提议代表黄单胞菌科中的一个新属和种，建议将其命名为 Chiayiivirga flava gen. nov.，sp. nov.。Chiayiivirga flava 的模式株为 CC-YHH031(T)（=BCRC 80274(T)=DSM 24163(T)）。
