Weon Hang-Yeon, Yoo Seung-Hee, Kim Yoo-Jeong, Lee Chang-Muk, Kim Byung-Yong, Jeon Young-Ah, Hong Seung-Beom, Anandham Rangasamy, Kwon Soon-Wo
Mushroom Research Division, National Institute of Horticultural and Medicinal Crop, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2308-12. doi: 10.1099/ijs.0.005165-0. Epub 2009 Jul 20.
A yellow-pigmented, Gram-negative, aerobic, rod-shaped bacterium, strain KIS3-4T, was isolated from soil collected on Daechung Island in the West Sea of Korea. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain KIS3-4T in a distinct lineage in the family Xanthomonadaceae. Strain KIS3-4T shared 87.3-93.7% sequence similarity with members of the family Xanthomonadaceae, and was related most closely to the genera Dyella and Dokdonella. In its biochemical characteristics, strain KIS3-4T was clearly separable from other genera within the family Xanthomonadaceae on the basis of the hydrolysis of cellulose and urea, high G+C content (64 mol%) and fatty acid profile. Major fatty acids (>10% of the total fatty acids) were iso-C17:1omega9c (32.8%), iso-C17:0 (18.0%) and iso-C16:0 (12.7%). Q-8 was the predominant respiratory quinone. Phosphatidylethanolamine and several unidentified aminophospholipids and phospholipids were present. Based on its unique phenotypic, genotypic and phylogenetic features, strain KIS3-4T represents a novel genus and species, for which the name Rudaea cellulosilytica gen. nov., sp. nov. is proposed. The type strain of Rudaea cellulosilytica is KIS3-4T (=KACC 12734T=JCM 15422T).
从韩国西海大川岛采集的土壤中分离出一株黄色色素、革兰氏阴性、需氧、杆状细菌，菌株KIS3-4T。基于16S rRNA基因序列的系统发育分析将菌株KIS3-4T置于黄单胞菌科的一个独特谱系中。菌株KIS3-4T与黄单胞菌科成员的序列相似性为87.3%-93.7%，与Dyella属和Dokdonella属关系最为密切。在其生化特性方面，菌株KIS3-4T基于纤维素和尿素的水解、高G+C含量（64 mol%）和脂肪酸谱，可与黄单胞菌科内的其他属明显区分。主要脂肪酸（占总脂肪酸的>10%）为异-C17:1ω9c（32.8%）、异-C17:0（18.0%）和异-C16:0（12.7%）。Q-8是主要的呼吸醌。存在磷脂酰乙醇胺和几种未鉴定的氨基磷脂和磷脂。基于其独特的表型、基因型和系统发育特征，菌株KIS3-4T代表一个新的属和种，为此提出了Rudaea cellulosilytica gen. nov., sp. nov.这个名称。Rudaea cellulosilytica的模式菌株是KIS3-4T（=KACC 12734T=JCM 15422T）。
