Indramanee Somsiri, Silsirivanit Atit, Pairojkul Chawalit, Wongkham Chaisiri, Wongkham Sopit
Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand.
Asian Pac J Cancer Prev. 2012;13 Suppl:119-24.
Cholangiocarcinoma (CCA) is an aggressive malignant tumor which is difficult to diagnose at an early stage. Because no reliable CCA specific markers are available at present, most patients are diagnosed after late clinical presentation. In many tumors, aberrant glycans participate in various steps of pathogenesis and progression. In this study, we investigated aberrant glycosylation in CCA tissues using lectin histochemistry to allow associations of specific glycans with clinicopathological features of the patients to be investigated. For this purpose, 14 lectins specific to 5 main glycan structures were used for screening. Nine lectins showed positive staining in hepatocyte sand stromal cells in liver tissues whereas three lectins, sWGA, SJA and UEA-I, had negative lectin binding to hepatocytes and normal bile duct epithelia but exhibited positive staining with CCA. sWGA was selected for further evaluation of (β-D-GlcNAc)n-glycoconjugate expressions in 44 CCA tissues. We found that sWGA- specific glycans were aberrantly expressed along with CCA development and the level of expression varied with histological types. It was highly expressed in papillary and well-differentiated types but was significantly reduced in poorly-differentiated lesions. Specific associations of sWGA-specific glycoconjugate expression with clinicopathological features or overall survival of patients were not apparent in this cohort study.
胆管癌（CCA）是一种侵袭性恶性肿瘤，早期难以诊断。由于目前尚无可靠的CCA特异性标志物，大多数患者在出现晚期临床表现后才被诊断出来。在许多肿瘤中，异常聚糖参与发病机制和进展的各个阶段。在本研究中，我们使用凝集素组织化学研究CCA组织中的异常糖基化，以研究特定聚糖与患者临床病理特征之间的关联。为此，使用了针对5种主要聚糖结构的14种凝集素进行筛选。9种凝集素在肝组织的肝细胞和基质细胞中呈阳性染色，而3种凝集素，即sWGA、SJA和UEA-I，与肝细胞和正常胆管上皮细胞的凝集素结合呈阴性，但在CCA中呈阳性染色。选择sWGA进一步评估44例CCA组织中（β-D-GlcNAc）n-糖缀合物的表达。我们发现，sWGA特异性聚糖随着CCA的发展而异常表达，表达水平因组织学类型而异。它在乳头状和高分化类型中高度表达，但在低分化病变中显著降低。在这项队列研究中，sWGA特异性糖缀合物表达与患者临床病理特征或总生存期之间没有明显的特定关联。
