School of Pathology, Institute of Medicine, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
Liver Fluke and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
J Int Med Res. 2021 Feb;49(2):300060520976864. doi: 10.1177/0300060520976864.
This study aimed to investigate the expression of -linked glycoprotein glycans in tissue of patients with cholangiocarcinoma compared with adjacent normal tissue.
Sixty patients with cholangiocarcinoma were included in the study. Permethylated -linked glycans from intrahepatic cholangiocarcinoma tissue and adjacent normal tissue were analyzed using nano-spray ionization-linear ion trap mass spectrometry. Histochemistry of peanut agglutinin lectin was used for detection and localization of galactose (Gal) 1, N-acetyl-galactosamine (GalNAc) 1.
-linked glycans from patients with cholangiocarcinoma were composed of di- to hexa-saccharides with a terminal galactose and sialic acids (N-acetylneuraminic acid [NeuAc]). A total of eight -linked glycan structures were detected. Gal1GalNAc1 and Gal2 N-acetyl-glucosamine 1 GalNAc1 expression was significantly higher in tissue from patients with cholangiocarcinoma compared with adjacent normal tissue, while NeuAc1Gal1GalNAc1 expression was significantly lower. High Gal1GalNAc1 expression was significantly associated with the late stage of cholangiocarcinoma (stages II-IV), lymphatic invasion, and vascular invasion.
Our study shows expression of -linked glycans in progression of cholangiocarcinoma and highlights the association of Gal1GalNAc1 with lymphatic and vascular invasion of cholangiocarcinoma.
本研究旨在比较胆管癌患者组织与邻近正常组织中β-连接糖蛋白聚糖的表达。
本研究纳入了 60 例胆管癌患者。采用纳升喷雾电离-线性离子阱质谱法分析肝内胆管癌组织和邻近正常组织中的甲氧基化β-连接聚糖。花生凝集素组织化学用于检测和定位半乳糖（Gal）1、N-乙酰半乳糖胺（GalNAc）1。
胆管癌患者的β-连接聚糖由二至六糖组成，末端为半乳糖和唾液酸（N-乙酰神经氨酸[NeuAc]）。共检测到 8 种β-连接糖链结构。与邻近正常组织相比，胆管癌组织中 Gal1GalNAc1 和 Gal2 N-乙酰-葡萄糖胺 1GalNAc1 的表达显著升高，而 NeuAc1Gal1GalNAc1 的表达显著降低。Gal1GalNAc1 高表达与胆管癌晚期（Ⅱ-Ⅳ期）、淋巴管浸润和血管浸润显著相关。
本研究表明β-连接聚糖在胆管癌进展过程中有表达，并强调了 Gal1GalNAc1 与胆管癌的淋巴管和血管浸润有关。
