Maisonneuve-Rosemont Hospital Research Center, University of Montreal, 5415 Boulevard de l'Assomption, Montréal, QC, Canada H1T 2M4.
Biomed Res Int. 2013;2013:126189. doi: 10.1155/2013/126189. Epub 2012 Dec 30.
Following activation, naïve CD8(+) T cells will differentiate into effectors that differ in their ability to survive: some will persist as memory cells while the majority will die by apoptosis. Signals given by antigen-presenting cells (APCs) at the time of priming modulate this differential outcome. We have recently shown that, in opposition to dendritic cell (DC), CD40-activated B-(CD40-B) cell vaccination fails to efficiently produce CD8(+) memory T cells. Understanding why CD40-B-cell vaccination does not lead to the generation of functional long-lived memory cells is essential to define the signals that should be provided to naïve T cells by APCs. Here we show that CD40-B cells produce very low amount of IL-6 when compared to DCs. However, supplementation with IL-6 during CD40-B-cell vaccination did not improve memory generation. Furthermore, IL-6-deficient DCs maintained the capacity to promote the formation of functional CD8(+) effectors and memory cells. Our results suggest that in APC vaccination models, IL-6 provided by the APCs is dispensable for proper CD8(+) T-cell memory generation.
在激活后，幼稚 CD8(+)T 细胞将分化为效应细胞，其存活能力不同：一些将作为记忆细胞持续存在，而大多数将通过细胞凋亡而死亡。在引发时由抗原呈递细胞 (APC) 发出的信号调节这种差异结果。我们最近表明，与树突状细胞 (DC) 相反，CD40 激活的 B 细胞（CD40-B）疫苗接种不能有效地产生 CD8(+)记忆 T 细胞。了解为什么 CD40-B 细胞疫苗接种不会导致功能性长寿记忆细胞的产生对于确定 APC 应该向幼稚 T 细胞提供的信号至关重要。在这里，我们表明与 DC 相比，CD40-B 细胞产生的 IL-6 量非常低。然而，在 CD40-B 细胞疫苗接种期间补充 IL-6并没有改善记忆的产生。此外，缺乏 IL-6 的 DC 仍然能够促进功能性 CD8(+)效应细胞和记忆细胞的形成。我们的结果表明，在 APC 疫苗接种模型中，APC 提供的 IL-6 对于适当的 CD8(+)T 细胞记忆的产生是可有可无的。
