Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, China.
Biomed Res Int. 2013;2013:301540. doi: 10.1155/2013/301540. Epub 2012 Dec 26.
Bone marrow stem cells (BMSCs) have the capacity to differentiate into mature cell types of multiple tissues. Thus, they represent an alternative source for organ-specific cell replacement therapy in degenerative diseases. In this study, we demonstrated that female rat BMSCs could differentiate into steroidogenic cells with the capacity for de novo synthesis of Estradiol-17 β (E2) under high glucose culture conditions with or without retinoic acid (RA). The cultured BMSCs could express the mRNA and protein for P450arom, the enzyme responsible for estrogen biosynthesis. Moreover, radioimmunoassay revealed that BMSCs cultured in the present culture system produced and secreted significant amounts of testosterone, androstenedione, and E2. In addition, RA promoted E2 secretion but did not affect the levels of androgen. These results indicate that BMSCs can synthesize and release E2 and may contribute to autologous transplantation therapy for estrogen deficiency.
骨髓基质细胞（BMSCs）具有分化为多种组织成熟细胞类型的能力。因此，它们代表了退行性疾病中器官特异性细胞替代治疗的另一种来源。在这项研究中，我们证明了雌性大鼠 BMSCs 在高葡萄糖培养条件下，无论是否存在视黄酸（RA），都可以分化为具有从头合成雌二醇-17β（E2）能力的甾体生成细胞。培养的 BMSCs 可以表达 P450arom 的 mRNA 和蛋白质，该酶负责雌激素的生物合成。此外，放射免疫测定显示，在本培养系统中培养的 BMSCs 产生和分泌大量的睾酮、雄烯二酮和 E2。此外，RA 促进了 E2 的分泌，但不影响雄激素的水平。这些结果表明 BMSCs 可以合成和释放 E2，可能有助于雌激素缺乏的自体移植治疗。
