Departamento de Biología Celular, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1, Colonia Sección 16, Tlalpan, 14080 México, DF, Mexico.
Biomed Res Int. 2013;2013:382058. doi: 10.1155/2013/382058. Epub 2013 Jan 14.
Particulate matter (PM) and nanoparticles (NPs) induce activation and dysfunction of endothelial cells characterized by inhibition of proliferation, increase of adhesion and adhesion molecules expression, increase of ROS production, and death. DHEA has shown anti-inflammatory and antioxidant properties in HUVEC activated with proinflammatory agents. We evaluated if DHEA could protect against some inflammatory events produced by PM10 and TiO2 NPs in HUVEC. Adhesion was evaluated by a coculture with U937 cells, proliferation by crystal violet staining, and oxidative stress through DCFDA and Griess reagent. PM10 and TiO2 NPs induced adhesion and oxidative stress and inhibited proliferation of HUVEC; however, when particles were added in combination with DHEA, the effects previously observed were abolished independently from the tested concentrations and the time of addition of DHEA to the cultures. These results indicate that DHEA exerts significant anti-inflammatory and antioxidative effects on the damage induced by particles in HUVEC, suggesting that DHEA could be useful to counteract the harmful effects and inflammatory diseases induced by PM and NPs.
颗粒物 (PM) 和纳米颗粒 (NPs) 可诱导内皮细胞的激活和功能障碍，其特征为增殖抑制、黏附增加和黏附分子表达增加、ROS 生成增加和死亡。DHEA 在被促炎剂激活的 HUVEC 中显示出抗炎和抗氧化特性。我们评估了 DHEA 是否可以防止 PM10 和 TiO2 NPs 在 HUVEC 中产生的一些炎症事件。通过与 U937 细胞共培养评估黏附，通过结晶紫染色评估增殖，通过 DCFDA 和 Griess 试剂评估氧化应激。PM10 和 TiO2 NPs 诱导 HUVEC 的黏附和氧化应激，并抑制其增殖；然而，当将颗粒与 DHEA 一起添加时，先前观察到的效果被消除，而与测试浓度和向培养物中添加 DHEA 的时间无关。这些结果表明，DHEA 对颗粒在 HUVEC 中引起的损伤具有显著的抗炎和抗氧化作用，表明 DHEA 可能有助于对抗 PM 和 NPs 引起的有害影响和炎症性疾病。
