感染甲型H5N1 A/鹅/克拉斯诺亚尔斯克/627/05病毒的小鼠肺部成纤维细胞的形态功能特征及纤维化形成

Morphofunctional characteristics of fibroblasts and fibrogenesis in the lungs of mice infected with influenza A/H5N1 A/goose/Krasnoozerskoye/627/05 virus.

作者信息

Anikina A G, Potapova O V, Shkurupy V A

机构信息

Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk.

出版信息

Bull Exp Biol Med. 2013 Jan;154(3):361-4. doi: 10.1007/s10517-013-1951-5.

DOI:10.1007/s10517-013-1951-5

PMID:23484201

Abstract

Mice C57Bl/6 were intranasally infected with influenza A/H5N1 A/goose/Krasnoozerskoye/627/05 virus. The expression of influenza A virus antigen in the lung interstitium fibroblasts was recorded during all periods of the study (on days 1-14 after infection), the maximum expression on days 1 and 3. On day 1, the volume density of collagen fibers in the lungs was 3-fold higher than in intact animals; by day 14 it increased almost 15-fold. The numerical density of PCNA(+)fibroblasts increased almost 2-fold from day 1 to day 10; "fibroplastic activity" of fibroblasts increased more than 3-fold.

摘要

将C57Bl/6小鼠经鼻内感染甲型H5N1流感病毒A/鹅/克拉斯诺奥泽尔斯克oye/627/05。在研究的所有阶段（感染后第1 - 14天）记录肺间质成纤维细胞中甲型流感病毒抗原的表达情况，在第1天和第3天表达量最高。第1天，肺中胶原纤维的体积密度比未感染动物高3倍；到第14天，其增加了近15倍。从第1天到第10天，增殖细胞核抗原（PCNA）阳性成纤维细胞的数量密度增加了近2倍；成纤维细胞的“纤维形成活性”增加了3倍多。