Zhu W, Zhu X, Huang G T-J, Cheung G S P, Dissanayaka W L, Zhang C
Department of General Dentistry, School and Hospital of Stomatology, Peking University, Beijing, China.
Int Endod J. 2013 Oct;46(10):962-70. doi: 10.1111/iej.12087. Epub 2013 Mar 14.
To investigate the new tissues growing into the pulp space of immature dog teeth that were infected, disinfected and filled with blood clot (BC), dental pulp cells (DPCs), platelet-rich plasma (PRP) or a combination of DPCs and PRP in immature dog teeth with apical periodontitis.
Fifty-six immature roots from mandibular premolars of four beagles were divided into four experimental groups (n = 40) and two control groups. After the induction of apical periodontitis, the root canals of experimental groups were disinfected with NaOCl irrigation and a tri-antibiotic paste medication. The canals were then filled with different materials according to the experimental group: BC group, DPCs group, PRP group or DPCs + PRP group. Access cavities were sealed with MTA and composite. Radiographs were taken after 90 days, and the jaws including the teeth were processed for histologic analysis. The data were statistically analysed using chi-square evaluation and Student's t-test.
Radiographic analyses demonstrated no significant difference between experimental groups in periradicular bone healing (P > 0.05), whilst those groups that used DPCs produced a significantly greater root thickening (P < 0.01). The histologic evaluation showed that the groups with PRP formed more tissues in the canals (P = 0.01). The groups with DPCs had substantially more mineralized tissue formation in the canal than those without DPCs, especially in the apical third. In DPCs + PRP group, bone-like tissue grew into the canal space from the periapical tissue.
A combination of DPCs + PRP increased vital tissue regeneration within the root canals of immature teeth associated with apical periodontitis.
研究在患有根尖周炎的未成熟犬牙中，新组织生长到感染、消毒并填充有血凝块（BC）、牙髓细胞（DPCs）、富血小板血浆（PRP）或DPCs与PRP组合的牙髓腔中的情况。
将4只比格犬下颌前磨牙的56个未成熟牙根分为4个实验组（n = 40）和2个对照组。诱导根尖周炎后，实验组的根管用次氯酸钠冲洗液和三联抗生素糊剂进行消毒。然后根据实验组不同，用不同材料填充根管：BC组、DPCs组、PRP组或DPCs + PRP组。用MTA和复合树脂封闭髓腔。90天后拍摄X线片，并对包含牙齿的颌骨进行组织学分析。数据采用卡方检验和学生t检验进行统计学分析。
X线片分析显示，实验组之间根尖周骨愈合无显著差异（P > 0.05），而使用DPCs的组牙根增厚明显更大（P < 0.01）。组织学评估显示，PRP组在根管内形成的组织更多（P = 0.01）。与未使用DPCs的组相比，使用DPCs的组在根管内形成的矿化组织明显更多，尤其是在根尖三分之一处。在DPCs + PRP组中，类骨组织从根尖周组织长入根管空间。
DPCs + PRP的组合增加了与根尖周炎相关的未成熟牙齿根管内的活性组织再生。
