Wu J Y, Sun Y X, Wang A B, Che G Y, Hu T J, Zhang X M
Jilin Province Key Laboratory of Animal Embryo Engineering, The Center for Animal Embryo Engineering of Jilin Province, College of Veterinary Medicine, Jilin University, Changchun, China; Department of Laboratory Medicines, The 90th Hospital of Jinan, Jinan, China.
Andrologia. 2014 Apr;46(3):308-12. doi: 10.1111/and.12084. Epub 2013 Mar 12.
Bovine serum is widely used for cryopreservation of various cells and tissues. However, its cryoprotective effects on the cells and tissues are ambiguous and controversial. To test the effects of newborn calf serum (NCS) on cryopreservation of bovine testis tissue, NCS of 0%, 5%, 10% and 20% (v/v) was added into minimum essential medium + 10% dimethyl sulphoxide (DMSO)-based medium according to our previous report. Interestingly, the testicular cell viabilities and spermatogonia percentages from four groups were very close. The results indicated that an increase in the concentration of NCS in freezing medium to 20% has no significant effect on survival of both testicular cells and spermatogonia, and 10% DMSO-based freezing medium can maintain the testicular cell viability and spermatogonia percentage at a relatively high level (83.4 ± 0.7 and 56.5 ± 2.2 respectively). Taken together, NCS is dispensable for cryopreservation of adult bovine testis tissue. Our results provide an evidence for cutting down the costs in cryopreservation research of bovine testis tissue by reducing or giving up the use of serum.
牛血清被广泛用于各种细胞和组织的冷冻保存。然而，其对细胞和组织的冷冻保护作用尚不明确且存在争议。为了测试新生牛血清（NCS）对牛睾丸组织冷冻保存的影响，根据我们之前的报告，将0%、5%、10%和20%（v/v）的NCS添加到基于最低限度基本培养基+10%二甲基亚砜（DMSO）的培养基中。有趣的是，四组的睾丸细胞活力和精原细胞百分比非常接近。结果表明，冷冻培养基中NCS浓度增加到20%对睾丸细胞和精原细胞的存活均无显著影响，且基于10%DMSO的冷冻培养基可将睾丸细胞活力和精原细胞百分比维持在相对较高水平（分别为83.4±0.7和56.5±2.2）。综上所述，NCS对于成年牛睾丸组织的冷冻保存并非必需。我们的结果为通过减少或放弃使用血清来降低牛睾丸组织冷冻保存研究成本提供了证据。
